Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lysine-PEGylation of Moroctocog Alfa: Purification and Activity Analysis.

Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion·2026
Same author

CRISPR/Cas9-mediated knockout of TopBP1 shifts the Bax/Bcl-2 balance toward apoptosis in MCF7 breast cancer cells.

Molecular biology reports·2026
Same author

Novel benzothiazole-indole acetamides as potential anticancer agents: synthesis, biological evaluation, and <i>in silico</i> studies.

RSC advances·2026
Same author

Finding and computational analyses of a novel mutation in CEP78 linked to cone-rod dystrophy and hearing loss.

Human molecular genetics·2026
Same author

Design and in silico evaluation of an mRNA vaccine against HTLV-1 using AI-driven reverse vaccinology approaches.

PloS one·2026
Same author

HLA Type and the Effect of HLA Antibodies in Kidney, Liver, and Pancreas Transplantation: A Review.

Avicenna journal of medical biotechnology·2026

Related Experiment Video

Updated: Jul 27, 2025

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

18.1K

Finding and engineering the newly found bacterial superoxide dismutase enzyme to increase its thermostability and

Saha Satvati1, Younes Ghasemi2,3, Sohrab Najafipour1,4

  • 1Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran.

Archives of Microbiology
|June 8, 2023
PubMed
Summary

This study identified a bacterial superoxide dismutase (SOD) with reduced allergenicity for commercial use. A specific mutation (K144A) enhanced enzyme stability and decreased immunogenicity, making it a promising antioxidant candidate.

Keywords:
Anoxybacillus gonensisEnzyme expressionIn silicoMutagenesis

More Related Videos

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
10:14

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Published on: November 8, 2019

6.4K
Evaluating the Immune Response of a Nanoemulsion Adjuvant Vaccine Against Methicillin-Resistant Staphylococcus aureus MRSA Infection
07:32

Evaluating the Immune Response of a Nanoemulsion Adjuvant Vaccine Against Methicillin-Resistant Staphylococcus aureus MRSA Infection

Published on: September 1, 2023

1.9K

Related Experiment Videos

Last Updated: Jul 27, 2025

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

18.1K
Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
10:14

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Published on: November 8, 2019

6.4K
Evaluating the Immune Response of a Nanoemulsion Adjuvant Vaccine Against Methicillin-Resistant Staphylococcus aureus MRSA Infection
07:32

Evaluating the Immune Response of a Nanoemulsion Adjuvant Vaccine Against Methicillin-Resistant Staphylococcus aureus MRSA Infection

Published on: September 1, 2023

1.9K

Area of Science:

  • Biochemistry
  • Enzymology
  • Protein Engineering

Background:

  • Superoxide dismutase (SOD) is a crucial antioxidant enzyme mitigating cellular oxidative stress.
  • Bacterial SODs are commercially valuable in cosmetics and pharmaceuticals but pose risks due to potential allergenicity.
  • Reducing the immunogenicity of bacterial SODs is essential for safe application.

Purpose of the Study:

  • To identify a suitable bacterial SOD source with decreased immunogenicity.
  • To engineer a modified SOD enzyme with enhanced stability and reduced allergenic potential.
  • To evaluate the efficacy and safety of the engineered SOD for industrial applications.

Main Methods:

  • Bioinformatic analysis of SOD sequences from five thermophilic bacteria.
  • In silico prediction of linear and conformational B-cell epitopes and allergenic features.
  • Site-directed mutagenesis (K144A) and recombinant protein expression in E. coli.
  • SDS-PAGE analysis, enzyme activity assays, and in silico immunogenicity assessment.

Main Results:

  • Anoxybacillus gonensis identified as a suitable SOD source based on BLAST, physicochemical, and allergenic predictions.
  • Five potential mutagenesis sites identified, with K144A showing promise.
  • The K144A mutation significantly increased enzyme stability and reduced predicted immunogenicity.
  • Recombinant K144A SOD exhibited an activity of 240 U/ml at room temperature.

Conclusions:

  • The engineered K144A SOD from Anoxybacillus gonensis demonstrates improved stability and reduced immunogenicity.
  • This modified enzyme represents a safer and more effective antioxidant for cosmetic and pharmaceutical applications.
  • In silico validation confirms the non-antigenic nature of the mutated protein.