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Updated: Jul 27, 2025
Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation
Published on: May 20, 2020
Development of a visual detection method for Salmonella based on loop-mediated isothermal amplification using
Wenbo Liu1, Shuai Zhang1, Chang Chen1
1Beijing Laboratory of Food Quality and Safety, Beijing Key Laboratory of Detection and Control of Spoilage Organisms and Pesticide Residues in Agricultural Products, College of Food Science and Engineering, Beijing University of Agriculture, No. 7 Bei Nong Road, ChangPing District, Beijing 102206, China.
A new visual detection method using loop-mediated isothermal amplification (LAMP) allows for rapid Salmonella detection in food. This method enhances food safety by quickly identifying viable Salmonella bacteria.
Area of Science:
- Food Safety
- Microbiology
- Molecular Diagnostics
Background:
- Salmonella is a significant food-borne pathogen impacting public health.
- Rapid and accurate detection of Salmonella is crucial for food safety protocols.
Purpose of the Study:
- To develop a rapid, visual detection strategy for viable Salmonella.
- To optimize and validate a loop-mediated isothermal amplification (LAMP) assay for Salmonella detection.
Main Methods:
- Designed Salmonella-specific primers targeting the phoP gene.
- Optimized loop-mediated isothermal amplification (LAMP) conditions with thermal inorganic pyrophosphatase.
- Utilized an ammonium molybdate chromogenic buffer for visual detection.
Main Results:
- Achieved a limit of detection of 1.83 × 10^2 CFU/reaction for viable Salmonella.
- Demonstrated high specificity with no nonspecific amplification observed.
- Showcased effective detection rates (89.11%-94.80%) in spiked apple juice samples.
Conclusions:
- The developed visual LAMP assay is a sensitive and specific method for detecting viable Salmonella.
- This strategy is suitable for the rapid detection of Salmonella in actual food samples, enhancing food safety.
- The method offers a promising tool for routine food safety monitoring.
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