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Related Experiment Videos

In vivo antidiarrheal activity of the crude extract and solvent fractions of Rhamnus prinoides (Rhamnaceae) leaves.

Teklie Mengie Ayele1, Endeshaw Chekol Abebe2, Zelalem Tilahun Muche3

  • 1Department of Pharmacy, College of Health Sciences, Debre Tabor University, Debre Tabor, Ethiopia.

Heliyon
|June 9, 2023
PubMed
Summary

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Rhamnus prinoides leaves demonstrated significant anti-diarrheal effects in vivo, reducing stool frequency and intestinal motility. These findings support its traditional use as a natural remedy for diarrhea.

Area of Science:

  • Pharmacology
  • Ethnobotany
  • Natural Product Chemistry

Background:

  • Conventional anti-diarrheal agents possess toxicities, necessitating research into safer alternatives.
  • Traditional medicine often holds potential for discovering effective and less toxic therapeutic compounds.

Purpose of the Study:

  • To investigate the in vivo anti-diarrheal activity of Rhamnus prinoides leaf extracts and fractions.
  • To scientifically validate the traditional use of Rhamnus prinoides for treating diarrhea.

Main Methods:

  • Crude methanol extract and solvent fractions of Rhamnus prinoides leaves were prepared.
  • In vivo anti-diarrheal activity was assessed using castor oil-induced diarrhea, enteropolling, and intestinal transit models in mice.
  • Statistical analysis was performed using one-way ANOVA and Tukey's post-test.
Keywords:
Anti enteropoolingCastor oil-induced diarrheaIntestinal transitRhamnus prinoides

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Main Results:

  • The methanol crude extract significantly reduced diarrhea frequency, intestinal motility, and fluid accumulation in a dose-dependent manner.
  • The 400 mg/kg methanol crude extract showed efficacy comparable to the standard drug, loperamide.
  • The n-butanol fraction (n-BF) also exhibited significant anti-diarrheal effects, including delaying onset and reducing motility.

Conclusions:

  • Rhamnus prinoides leaf extracts and fractions possess significant in vivo anti-diarrheal properties.
  • The study validates the traditional application of Rhamnus prinoides in managing diarrhea.
  • Further research into the specific active compounds and mechanisms of action is warranted.