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Updated: Jul 27, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
Published on: March 15, 2019
Genome sequencing of
Nashwa Tarek1,2, Ahmed F Azmy3, Ahmed S Khairalla3,4
1Department of Botany and Microbiology, Faculty of Science, Beni-Suef University, Egypt.
Objective:
This study aimed to isolate and investigate a bacterium from an Egyptian adult's healthy oral cavity, focusing on its probiotic properties, especially its antagonistic activity against oral pathogens.
Methods:
The isolated bacterium NT04 using 16S rRNA gene sequencing, was identified as Enterococcus faecium. In this study, the whole genome of Enterococcus faecium NT04 was sequenced and annotated by bioinformatics analysis tools.
Results:
Numerous genes encoding the production of diverse metabolic and probiotic properties, such as bacteriocin-like inhibitory substances (Enterocin A and B), cofactors, antioxidants, and vitamins, were confirmed by genomic analysis. There were no pathogenicity islands or plasmid insertions found. This strain is virulent for host colonization rather than invasion.
Conclusion:
Genomic characteristics of strain NT04 support its potentiality as an anti-oral pathogen probiotic candidate.
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