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Published on: May 18, 2022
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The Prolonged Terminal Phase of Human Life Induces Survival Response in the Skin Transcriptome
Biorxiv : the Preprint Server for Biology
|June 9, 2023
Summary
Human death triggers cellular survival pathways in skin, especially after slow death. Dermal fibroblasts show resilience, while skin
Area of Science:
- Molecular Biology
- Genomics
- Cellular Biology
Background:
- Human death is a complex biological event.
- Cellular survival post-mortem varies based on the death process.
- Understanding postmortem cellular adaptation is crucial for interpreting biological data.
Approach:
- Investigated postmortem gene expression in 701 human skin samples using RNA sequencing.
- Analyzed the impact of terminal phase duration on cellular survival pathways.
- Utilized single nucleus RNA-sequencing to identify resilient cell types.
Key Points:
- Longer terminal phases (slow death) induce survival pathways like PI3K-Akt signaling in postmortem skin.
- Embryonic developmental transcription factors (FOXO1, FOXO3, ATF4, CEBPD) were upregulated.
- Dermal fibroblasts demonstrated resilience via PI3K-Akt signaling; endothelial cells showed angiogenesis.
- Skin-specific functional pathways (melanogenesis, ECM) were downregulated.
Conclusions:
- Death is a biological variable influencing tissue transcriptomics.
- Slow death promotes cellular survival mechanisms in skin.
- Findings have implications for interpreting data from deceased individuals and transplant tissues.
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