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CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Related Experiment Video

Updated: Jul 27, 2025

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

Published on: September 10, 2021

CRISPR-based diagnostics detects invasive insect pests.

Pathour R Shashank^1,2, Brandon M Parker^1,3,4, Santosh R Rananaware⁵

¹McGuire Center for Lepidoptera and Biodiversity, Florida Museum of Natural History, University of Florida, Gainesville, FL 32611, USA.

Biorxiv : the Preprint Server for Biology

|June 9, 2023

Summary

This summary is machine-generated.

A new CRISPR-based diagnostic tool accurately identifies animal species using the CO1 gene. This rapid, lab-free method offers high sensitivity for detecting invasive pests and revolutionizing organism monitoring.

Keywords:

CRISPR Cas12a Keiferia lycopersicella Phthorimaea absoluta RPA Scrobipalpa atriplicella Tuta absoluta diagnostics genetic identification leaf miner

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Last Updated: Jul 27, 2025

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

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Digital-Droplet PCR to Detect Indels Mutations in Genetically Modified Anopheline Mosquito Populations

Digital-Droplet PCR to Detect Indels Mutations in Genetically Modified Anopheline Mosquito Populations

Published on: June 28, 2021

Area of Science:

Molecular Biology
Genetics
Ecology

Background:

Rapid organism identification is crucial for ecological monitoring, disease diagnosis, and pest control.
Existing methods can be slow, require laboratory settings, and lack sensitivity.

Approach:

Developed a CRISPR-based diagnostic assay targeting the universal cytochrome-oxidase 1 (CO1) gene in Animalia.
Combined recombinase polymerase amplification (RPA) with CRISPR for sensitive signal generation.
Validated the assay on three invasive moth species: Keiferia lycopersicella, Phthorimaea absoluta, and Scrobipalpa atriplicella.

Key Points:

Achieved 100% accuracy in identifying all tested moth species.
Demonstrated high sensitivity with detection limits as low as 120 fM for P. absoluta.
Assay is rapid (under one hour), lab-free, and reduces cross-contamination risk.

Conclusions:

This CRISPR-CO1 approach offers a revolutionary, highly accurate, and accessible method for animal detection and monitoring.
Potential applications span ecological studies, invasive species management, and disease surveillance.
The CO1 gene's universality allows for broad applicability across the Animalia kingdom.