The effects of lymph on lymphocyte migration in vivo

The in vivo administration of syngeneic lymph significantly modified lymphocyte migration in the mouse. When administered intravenously, lymph inhibited cell localization into lymph nodes, an effect that could be mimicked by lymphocyte pre-incubation with lymph in vitro. The unilateral intrafootpad injection of FR2 of lymph did enhance cell migration, both to the draining popliteal node and to the site of injection when compared to the contralateral foot or draining node. Furthermore, the subcutaneous transplant of polyurethane sponges soaked in lymph or serum into both flanks of a mouse led to increased migration and accumulation of cells in the sponges coated with lymph when compared to sponges treated with mouse serum alone.