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Josue Baeza1,2, Barbara E Coons3,2, Zongtao Lin4

  • 1Department of Biochemistry & Biophysics, University of Pennsylvania, Philadelphia, PA 19104.

Biorxiv : the Preprint Server for Biology
|June 9, 2023
PubMed
Summary
This summary is machine-generated.

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This study introduces a new in utero stable isotope labeling method to measure protein synthesis in developing mouse fetuses. This technique quantifies tissue-specific protein dynamics, revealing unique developmental signatures.

Area of Science:

  • Developmental Biology
  • Proteomics
  • Molecular Biology

Background:

  • Protein synthesis regulation is crucial for mammalian fetal development.
  • Defects in protein expression can cause severe developmental issues.
  • Quantitative methods for monitoring in utero protein synthesis are limited.

Approach:

  • Developed a novel in utero stable isotope labeling technique using labeled lysine and arginine.
  • Injected pregnant mice via the vitelline vein at various gestational days.
  • Analyzed tissue-specific nascent proteomes from harvested fetal organs (brain, liver, lung, heart).

Key Points:

  • Achieved a mean amino acid incorporation rate of 17.50 ± 0.6% across all organs.
  • Identified unique tissue signatures using hierarchical clustering of the nascent proteome.
Keywords:
fetal developmentin utero injectionprotein turnoverproteomicspulse labeling

Related Experiment Videos

  • Calculated proteome-wide turnover rates (kobs) ranging from 3.81E-5 to 0.424 hour-1.

    • Conclusions:

    • Observed similar protein turnover profiles but significantly different turnover rate distributions across organs.
    • Translational kinetic profiles revealed differentially expressed protein pathways and synthesis rates.
    • Findings correlate with known physiological changes during mouse development.