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Isolation and

Vida Štrancar1,2, Monika Marušić1, Jasmina Tušar1

  • 1Centre of Excellence for Biosensors, Instrumentation and Process Control, Ajdovščina, Slovenia.

Frontiers in Cellular and Infection Microbiology
|June 9, 2023
PubMed

Insights

Novel bacteriophages effectively target Staphylococcus epidermidis biofilms, offering a promising alternative to antibiotics for prosthetic joint infections. These phages show potential for preventing recurrent infections and reducing the need for revision surgery.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Bacteriophage Therapy

Background:

  • Staphylococcus epidermidis is a major cause of prosthetic joint infections, often forming biofilms that resist antibiotics.
  • Current treatments for these infections may require prolonged antibiotic courses or surgical intervention.
  • Bacteriophage therapy is being explored as a potential adjunctive or alternative treatment to combat antibiotic-tolerant S. epidermidis.

Purpose of the Study:

  • To isolate and characterize novel lytic bacteriophages targeting Staphylococcus epidermidis.
  • To evaluate the efficacy of these phages against S. epidermidis in planktonic and biofilm states.
  • To investigate the potential of phage therapy for prosthetic joint infections.

Main Methods:

  • Isolation and in vitro characterization of three novel lytic S. epidermidis phages.
  • Genome analysis to identify antibiotic resistance genes and virulence factors.
  • Testing phage efficacy against diverse clinical S. epidermidis strains, including those in biofilms.

Main Results:

  • Three novel lytic bacteriophages targeting S. epidermidis were successfully isolated and characterized.
  • Phage genomes were free of antibiotic resistance genes and virulence factors.
  • The isolated phages demonstrated efficacy against a broad range of S. epidermidis strains, including those forming biofilms.

Conclusions:

  • Novel lytic phages are effective against S. epidermidis, including biofilm-forming strains.
  • These phages represent a promising therapeutic strategy for prosthetic joint infections.
  • Phage therapy could offer an alternative or supplement to antibiotics, preventing infection relapse.
Keywords:
Staphylococcus epidemidisbiofilmsphage safety and efficacyprosthetic joint infectionstherapeutic phages

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