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Related Experiment Video

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Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Complete genome sequencing of

Valeria Valenzuela Ruiz¹, Gustavo Santoyo², Lorena Jacqueline Gómez Godínez³

¹Instituto Tecnológico de Sonora (ITSON), 5 de febrero 818 Sur, C.P. 85000, Col. Centro, Cd. Obregón, Sonora, Mexico.

Current Research in Microbial Sciences

|June 9, 2023

Summary

The complete genome of Bacillus cabrialesii TE3T, a plant growth-promoting bacterium, was sequenced. This analysis identified genes for agricultural applications, including biological control and antimicrobial properties, supporting its use in sustainable agriculture.

Keywords:

Biological control agent Food security Lipopeptides Plant growth-promoting bacteria Whole genome sequencing

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Area of Science:

Microbiology
Genomics
Agricultural Science

Background:

Bacillus cabrialesii TE3T is a Gram-positive bacterium known for promoting plant growth and acting as a biological control agent.
Understanding its genetic makeup is crucial for optimizing its agricultural applications.

Purpose of the Study:

To present the complete, circularized genome sequence of the Bacillus cabrialesii TE3T type strain.
To identify genes and biosynthetic clusters related to plant growth promotion, biological control, and antimicrobial activities.

Main Methods:

A hybrid genome assembly approach was employed, combining Illumina MiSeq short-read and Oxford Nanopore Technology (ONT) MinION long-read sequencing.
Genome annotation was performed using RAST, Prokka (Rapid Prokaryotic Genome Annotation), and PGAP (Prokaryotic Genome Annotation Pipeline).
Biosynthetic gene clusters were identified using antiSMASH.

Main Results:

A closed circular chromosome of 4,125,766 bp with 44.2% G+C content was assembled.
The genome contains 4,282 Coding DNA Sequences (CDS), with 4 related to plant growth promotion and 28 to biological control.
Seven putative biosynthetic gene clusters, including Fengycin, Bacilysin, and Surfactin, associated with antimicrobial and antifungal properties, were identified.

Conclusions:

The complete genome sequence of Bacillus cabrialesii TE3T reveals significant genetic potential for agricultural applications.
The identified genes and clusters support the bacterium's bioactivities, making it a promising candidate for developing bio-based inoculants for sustainable agriculture.