Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Diabetes Mellitus: Overview and Type I Subtype01:22

Diabetes Mellitus: Overview and Type I Subtype

2.8K
Diabetes mellitus is a chronic metabolic disorder characterized by high blood glucose levels due to inadequate insulin production, insulin resistance, or both. The condition affects millions worldwide and can significantly impact their health and quality of life.
Type 1 diabetes is an autoimmune disease in which the immune system mistakenly attacks and destroys the insulin-producing beta cells in the pancreas. As a result, the body is unable to produce sufficient insulin, and individuals with...
2.8K
Diabetes: Symptoms, Diagnosis, and Complications01:15

Diabetes: Symptoms, Diagnosis, and Complications

605
For most patients, experiencing several weeks of polyuria, polydipsia, fatigue, and significant weight loss may indicate the presence of diabetes. Furthermore, adults displaying the phenotypic appearance of type 2 diabetes (particularly those who are obese and not initially insulin-requiring), may have islet cell autoantibodies, suggesting autoimmune-mediated β cell destruction and a diagnosis of latent autoimmune diabetes of adults (LADA). The categorization of glucose homeostasis is...
605
Diabetes Mellitus: Type 2 and Gestational01:22

Diabetes Mellitus: Type 2 and Gestational

2.6K
Type 2 diabetes, characterized by insulin resistance, arises when the insulin receptors on cells lose responsiveness to insulin, diminishing the cell's capacity to take up glucose, resulting in elevated blood glucose levels. To receive a diagnosis of Type 2 diabetes, a series of blood glucose tests are necessary to assess whether the blood glucose falls within normal parameters. If the result is out of the normal range, a patient may be diagnosed as prediabetic or diabetic, depending on the...
2.6K
Pathophysiology of Diabetes01:20

Pathophysiology of Diabetes

1.0K
Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia. The four categories of diabetes are type 1 diabetes, type 2 diabetes, other specific types of diabetes, and gestational diabetes.
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility,...
1.0K
Proteoglycans01:05

Proteoglycans

4.0K
Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
4.0K
Complement System01:27

Complement System

2.5K
The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association of IgG N-Glycans With Adverse Outcomes in CKD.

Kidney international reports·2026
Same author

One-Year Comparative Outcomes of Conventional Versus Accelerated Corneal Cross-Linking in Progressive Keratoconus.

Medicina (Kaunas, Lithuania)·2026
Same author

Proinflammatory Epstein-Barr Virus Antibody Functions Track with Disease Activity in Multiple Sclerosis.

Annals of neurology·2026
Same author

Glycoproteomic and genetic analysis of N-glycosylation of complement component C3 reveals immune pathway regulation.

Genome biology·2026
Same author

Physical activity and IgG N-glycosylation in medical students: a cross-sectional study.

Croatian medical journal·2026
Same author

Perioperative changes in IgG and plasma N-glycosylation in children with acute appendicitis and elective surgery: a prospective study.

Croatian medical journal·2026

Related Experiment Video

Updated: Jul 27, 2025

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells
06:27

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

Published on: May 6, 2013

16.8K

Human complement component C3 N-glycome changes in type 1 diabetes complications.

Dinko Šoić1, Jerko Štambuk2, Marko Tijardović1

  • 1Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia.

Frontiers in Endocrinology
|June 9, 2023
PubMed
Summary
This summary is machine-generated.

Changes in complement component C3 N-glycosylation are linked to type 1 diabetes (T1D) complications like albuminuria and retinopathy. These N-glycan profiles may serve as novel biomarkers for T1D progression and severity.

Keywords:
LC-MSN-glycosylationcomplement component (C3)glycopeptidestype 1 diabetes complications

More Related Videos

Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid
06:21

Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid

Published on: April 7, 2023

1.6K
Extraction of Tissue Antigens for Functional Assays
08:32

Extraction of Tissue Antigens for Functional Assays

Published on: September 10, 2012

10.9K

Related Experiment Videos

Last Updated: Jul 27, 2025

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells
06:27

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

Published on: May 6, 2013

16.8K
Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid
06:21

Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid

Published on: April 7, 2023

1.6K
Extraction of Tissue Antigens for Functional Assays
08:32

Extraction of Tissue Antigens for Functional Assays

Published on: September 10, 2012

10.9K

Area of Science:

  • Biochemistry
  • Immunology
  • Endocrinology

Background:

  • N-glycosylation changes are implicated in various diseases and can serve as biomarkers.
  • Type 1 diabetes (T1D) is associated with altered serum N-glycans, particularly concerning disease complications.
  • Complement component C3 plays a role in diabetic nephropathy and retinopathy, with its N-glycome altered in T1D patients.

Purpose of the Study:

  • To investigate the associations between C3 N-glycan profiles and diabetic nephropathy (albuminuria) and retinopathy in T1D.
  • To explore the relationship between C3 N-glycome and other T1D complication risk factors, including hypertension, glycaemic control, and disease duration.

Main Methods:

  • Serum samples from 189 T1D patients were analyzed for C3 N-glycosylation profiles using a high-throughput method.
  • Linear modeling was employed to assess the interconnection of C3 N-glycome with T1D complications and risk factors like HbA1c, hypertension, smoking, eGFR, and disease duration.

Main Results:

  • Significant alterations in C3 N-glycome were observed in T1D patients with severe albuminuria and hypertension.
  • Most C3 glycopeptides were associated with HbA1c levels, and one glycoform changed in non-proliferative diabetic retinopathy.
  • Smoking and estimated glomerular filtration rate (eGFR) did not affect C3 N-glycome, which was also independent of disease duration.

Conclusions:

  • C3 N-glycosylation profiles are valuable in distinguishing T1D subjects with different complications.
  • The independence of these changes from disease duration suggests a potential link to disease onset.
  • C3 N-glycome represents a promising novel biomarker for T1D progression and severity.