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Pankaj Patyal1, Daniel Fil1, Patricia A Wight1

  • 1Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, Little Rock, AR, United States.

Frontiers in Cellular Neuroscience
|June 9, 2023
PubMed
Summary
This summary is machine-generated.

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Myelin proteolipid protein gene (Plp1) expression in the mouse intestine is highest during early development and concentrated in the duodenum. An enhancer region in intron 1 is crucial for regulating this Plp1 gene expression.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Molecular Biology

Background:

  • The myelin proteolipid protein gene (Plp1) is known to be expressed in the enteric nervous system (ENS) glia of mice.
  • Limited information exists regarding Plp1 expression patterns within the intestinal tract.

Purpose of the Study:

  • To investigate the spatiotemporal expression of Plp1 at both mRNA and protein levels in the mouse intestine across different developmental stages.
  • To identify regulatory elements controlling Plp1 expression in the intestine.

Main Methods:

  • Quantitative analysis of Plp1 mRNA and protein expression in mouse intestinal tissues at postnatal days 2, 9, 21, and 88.
  • Utilized a 6.2hPLP(+)Z/FL transgene as a reporter for Plp1 gene expression.
  • Investigated the role of the wmN1 enhancer region by creating a modified transgene lacking this element.
Keywords:
DM20enhancerenteric nervous system (ENS)gene expressionintestinemyelin proteolipid protein gene (Plp1)

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Main Results:

  • Plp1 expression is predominantly observed during early postnatal development, with the DM20 isoform being the primary form.
  • Western blot analysis revealed distinct migration patterns for intestinal vs. brain-derived PLP and DM20.
  • Transgene-driven reporter activity indicated highest Plp1 expression in the duodenum, decreasing along the intestinal axis.
  • Deletion of the wmN1 enhancer region significantly reduced both transgene mRNA levels and reporter activity, highlighting its regulatory role.

Conclusions:

  • Plp1 expression in the mouse intestine is developmentally regulated and localized, with a significant role for the wmN1 enhancer.
  • The findings suggest a conserved mechanism for Plp1 gene regulation across different nervous system compartments.