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Related Experiment Video

Updated: Jul 27, 2025

The Effect of Anti-Fatigue Decoction on the Behaviors and Serological Indicators in a Central Fatigue Rat Model
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Withania

Rokaya E Maarouf1, Khaled Shaaban Azab1, Neama M El Fatih1

  • 1Radiation Biology Department, Radiation Research Division, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.

Human & Experimental Toxicology
|June 9, 2023
PubMed
Summary

Withania somnifera (Ashwagandha) enhances apoptosis in breast cancer cells, both alone and when combined with radiation therapy. This natural product shows anti-proliferative effects by influencing key cell death pathways.

Keywords:
AshwagandhaBAXBCL-2MCF-7MDA-231SIRT-1γ-rays

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Area of Science:

  • Oncology
  • Pharmacology
  • Cell Biology

Background:

  • Natural products offer potential for cancer treatment with reduced side effects.
  • Withania somnifera (Ashwagandha) is explored for its anti-cancer properties.
  • Understanding its role in apoptosis and signaling pathways is crucial.

Purpose of the Study:

  • To assess Withania somnifera's (WS) role in inducing apoptosis in irradiated breast cancer cells (MCF7 and MDA-MB-231).
  • To investigate the involvement of the SIRT1-BCL2/Bax signaling pathway in WS-induced apoptosis.
  • To evaluate the combined effect of WS and gamma radiation on cancer cell death.

Main Methods:

  • Cell culture of MCF7 and MDA-MB-231 breast cancer lines.
  • Treatment groups included control, WS alone, gamma radiation (4 Gy) alone, and WS + gamma radiation.
  • Flow cytometry (Annexin V, cell cycle analysis) and gene expression analysis (SIRT1, BCL2, Bax) were performed.

Main Results:

  • Withania somnifera (WS) demonstrated anti-proliferative effects with determined IC50 values for both cell lines.
  • WS induced apoptosis and cell cycle arrest at specific phases (pre-G, G2/M, preG1) in MDA-MB-231 and MCF7 cells.
  • Combined WS and radiation treatment significantly upregulated SIRT1 and BCL2, while downregulating BAX expression.

Conclusions:

  • Withania somnifera (WS) exhibits anti-proliferative activity against MDA-MB-231 and MCF7 breast cancer cells.
  • WS enhances apoptosis, potentially through modulation of the SIRT1-BCL2/Bax pathway.
  • The combination of WS and radiation therapy may offer a synergistic approach to cancer treatment.