Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Venous Thrombosis II: Clinical Manifestations and Diagnostic Studies01:20

Venous Thrombosis II: Clinical Manifestations and Diagnostic Studies

16
The key difference between Superficial Vein Thrombosis (SVT) and Deep Vein Thrombosis (DVT) lies in their location and severity.Clinical ManifestationsSVT typically presents with localized pain, tenderness, and redness along the course of a superficial vein, often accompanied by a palpable, cord-like structure under the skin. This condition is usually less dangerous than DVT but can be uncomfortable and may lead to complications such as cellulitis or, rarely, a clot extension into the deep...
16
Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

314
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
314

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Response to: comment on "FOXL2 expression in dysgenetic gonads supports the diagnostic possibility of ovotesticular differences of sex development".

Virchows Archiv : an international journal of pathology·2026
Same author

Correction: Glioblastoma multiforme: insights into pathogenesis, key signaling pathways, and therapeutic strategies.

Molecular cancer·2026
Same author

<i>ESR1</i> mutations in ER-positive breast cancer: from endocrine resistance to ctDNA-guided therapeutic interception.

Exploration of targeted anti-tumor therapy·2026
Same author

Paleopathology Meets Public Health: Deep-Time Syndemics and the Ecology of Emerging Infections.

Pathogens (Basel, Switzerland)·2026
Same author

Expression of melanocytic markers in uterine smooth muscle tumours.

Histopathology·2026
Same author

FOXL2 expression in dysgenetic gonads supports the diagnostic possibility of ovotesticular differences of sex development.

Virchows Archiv : an international journal of pathology·2026

Related Experiment Video

Updated: Jul 27, 2025

Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue
06:36

Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue

Published on: May 1, 2019

7.1K

Routine elastin staining improves venous invasion detection in colorectal carcinoma.

Hisham F Bahmad1, Ferial Alloush1, Ali Salami2

  • 1Arkadi M. Rywlin M.D. Department of Pathology and Laboratory Medicine, Mount Sinai Medical Center, Miami Beach, FL 33140, USA.

Annals of Diagnostic Pathology
|June 9, 2023
PubMed
Summary

Routine elastin staining significantly increased venous invasion detection in colorectal cancer specimens. This method enhances the identification of tumor spread, improving diagnostic accuracy for metastatic progression.

Keywords:
Colorectal carcinomaElastinQuality improvementSmall vessel invasionStainVenous invasion

More Related Videos

Diagnosis of Neoplasia in Barrett&#8217;s Esophagus using Vital-dye Enhanced Fluorescence Imaging
06:55

Diagnosis of Neoplasia in Barrett’s Esophagus using Vital-dye Enhanced Fluorescence Imaging

Published on: May 11, 2014

12.1K
Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy
09:58

Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy

Published on: April 9, 2018

10.6K

Related Experiment Videos

Last Updated: Jul 27, 2025

Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue
06:36

Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue

Published on: May 1, 2019

7.1K
Diagnosis of Neoplasia in Barrett&#8217;s Esophagus using Vital-dye Enhanced Fluorescence Imaging
06:55

Diagnosis of Neoplasia in Barrett’s Esophagus using Vital-dye Enhanced Fluorescence Imaging

Published on: May 11, 2014

12.1K
Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy
09:58

Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy

Published on: April 9, 2018

10.6K

Area of Science:

  • Pathology
  • Oncology
  • Surgical Pathology

Background:

  • Colorectal carcinoma is a leading cause of cancer mortality in North America.
  • Tumor cell invasion into blood and lymphatic vessels is critical for colorectal carcinoma metastasis.

Purpose of the Study:

  • To evaluate the impact of routine elastin staining on venous invasion (VI) detection in colorectal carcinoma (CRC) resection specimens.
  • To compare VI detection rates before and after implementing a standardized elastin staining protocol.

Main Methods:

  • A before-and-after study design was employed, comparing CRC specimens from 2021 (pre-implementation, n=93) and 2022 (post-implementation, n=61).
  • Routine elastin staining was implemented on all feasible tumor-containing blocks in the post-implementation cohort.
  • Clinicopathological parameters were collected and analyzed for both cohorts.

Main Results:

  • Venous invasion detection increased significantly from 18.6% in the pre-implementation cohort to 50.8% in the post-implementation cohort.
  • Elastin staining implementation showed a significant association with increased VI detection (OR=4.5, p<0.001) on univariate analysis and remained independently significant (OR=2.6, p=0.034) on multivariate analysis.
  • No significant differences were observed in other clinicopathological parameters between the cohorts.

Conclusions:

  • Routine elastin staining on all tumor-containing blocks in colorectal carcinoma resection specimens confirms an increased rate of venous invasion detection.
  • This enhanced detection method improves the identification of a key factor in colorectal cancer metastasis.