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Related Experiment Video

Updated: Jul 27, 2025

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

Mid-infrared single-photon 3D imaging.

Jianan Fang¹, Kun Huang^2,3,4, E Wu^1,5

¹State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, China.

Light, Science & Applications

|June 9, 2023

Summary

This summary is machine-generated.

This study introduces a novel mid-infrared (MIR) time-of-flight imaging system with single-photon sensitivity. This breakthrough enables high-resolution 3D imaging even in extremely low-light conditions.

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Published on: December 1, 2016

Area of Science:

Photonics and Imaging Technologies
Biomedical Optics
Materials Science

Background:

Active mid-infrared (MIR) 3D imaging is crucial for biomedical and industrial applications.
Current limitations include the need for sensitive, fast MIR sensors for low-light imaging.

Purpose of the Study:

To develop a MIR time-of-flight imaging system with single-photon sensitivity and femtosecond timing resolution.
To overcome challenges in low-light level MIR 3D imaging.

Main Methods:

Utilized nonlinear frequency upconversion to optically gate back-scattered MIR photons with ultrashort pump pulses.
Employed a silicon camera for recording time-stamped, upconverted images.
Implemented a spatiotemporal correlation-based numerical denoiser for photon-starving conditions.

Main Results:

Achieved single-photon sensitivity and femtosecond timing resolution in MIR 3D imaging.
Demonstrated high lateral and depth resolutions for 3D reconstruction.
Successfully revealed object profiles and reflectivity at detected fluxes below 0.05 photons/pixel/second.

Conclusions:

The developed MIR 3D imager offers high detection sensitivity and precise timing resolution.
Its wide-field operation capabilities may advance research in life and material sciences.
This technology addresses critical challenges in low-light MIR 3D imaging.