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Effect of

Jinyong Zhou1,2, Keqin Zhang1,3, Jun Gao1,2

  • 1Institute of Animal Husbandry & Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.

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Summary
This summary is machine-generated.

Poria cocos mushroom polysaccharides (PCPs) improve Shanghai white pig sperm quality and reduce DNA methylation during cryopreservation. Optimal PCPs concentrations (600-900 μg/mL) enhance viability, motility, and antioxidant function, aiding boar semen preservation.

Keywords:
DNA methylationPoria cocos mushroom polysaccharides (PCPs)Shanghai white pigantioxidantsemen cryopreservation

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Area of Science:

  • Reproductive Biology
  • Animal Science
  • Biochemistry

Background:

  • Cryopreservation of boar spermatozoa is crucial for swine breeding programs.
  • Cryopreservation can negatively impact sperm quality and induce DNA methylation changes.
  • Poria cocos mushroom polysaccharides (PCPs) are bioactive compounds with potential antioxidant properties.

Purpose of the Study:

  • To investigate the effects of varying concentrations of PCPs on the quality of cryopreserved Shanghai white pig spermatozoa.
  • To assess the impact of PCPs on DNA methylation patterns in cryopreserved boar spermatozoa.
  • To determine the optimal concentration of PCPs for improving boar semen cryopreservation.

Main Methods:

  • Semen from eight Shanghai white pigs was collected and diluted with extenders containing different concentrations of PCPs (0-1500 μg/mL).
  • Cryopreserved spermatozoa were thawed and evaluated for viability, motility, plasma membrane integrity, acrosome integrity, and mitochondrial activity.
  • Antioxidant functions (ROS, MDA, GSH-Px, SOD, CAT) and DNA methylation (5-mC) levels were analyzed.

Main Results:

  • PCPs supplementation significantly improved sperm viability, motility, and plasma membrane integrity at concentrations of 600-1200 μg/mL.
  • Acrosome integrity and mitochondrial activity were enhanced by 600 and 900 μg/mL PCPs.
  • PCPs reduced oxidative stress markers (ROS, MDA) and modulated antioxidant enzyme activities (GSH-Px, SOD, CAT), while significantly decreasing 5-methylcytosine (5-mC) levels.

Conclusions:

  • Supplementation with 600-900 μg/mL PCPs effectively improves the quality of cryopreserved Shanghai white pig spermatozoa.
  • PCPs mitigate cryopreservation-induced DNA methylation in boar spermatozoa.
  • PCPs represent a promising strategy for enhancing boar semen cryopreservation protocols.