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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Related Experiment Video

Updated: Jul 11, 2026

Detection of Copy Number Alterations Using Single Cell Sequencing

Detection of Copy Number Alterations Using Single Cell Sequencing

Published on: February 17, 2017

Special Issue on Whole Genome Amplification.

Richard Jäger^1,2,3

¹Department of Natural Sciences, Bonn-Rhein-Sieg University of Applied Sciences, von-Liebig Str. 20, 53359 Rheinbach, Germany.

International Journal of Molecular Sciences

|June 10, 2023

Summary

Whole-genome amplification (WGA) enables genetic analysis from minimal DNA samples. This technique is crucial for studying genomes from single cells and viruses.

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Last Updated: Jul 11, 2026

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Area of Science:

Genomics
Molecular Biology
Biotechnology

Background:

Whole-genome amplification (WGA) techniques are essential for analyzing limited DNA quantities.
These methods allow for genome-wide studies using samples like single cells or virions.

Discussion:

WGA overcomes limitations in DNA availability for genetic research.
It facilitates comprehensive genomic analysis from minute biological samples.

Key Insights:

WGA provides a powerful tool for genetic analysis of scarce DNA.
Enables genome-wide studies on single cells (prokaryotic and eukaryotic) and virions.

Outlook:

Advancements in WGA will further expand possibilities in genetic research.
Potential applications include personalized medicine and infectious disease diagnostics.