Role of NPR1 in Systemic Acquired Stomatal Immunity
Qijie Guan1, Lisa David2, Riley Moran2
1Department of Biology, University of Mississippi, Oxford, MS 38677, USA.
Plants (Basel, Switzerland)
|June 10, 2023
Summary
Non-expressor of Pathogenesis Related 1 (NPR1) is crucial for plant stomatal immunity. The npr1-1 mutant shows impaired stomatal closure and increased pathogen entry, highlighting NPR1's role in plant defense.
Area of Science:
- Plant immunity
- Plant physiology
- Molecular plant-pathogen interactions
Background:
- Stomatal immunity is the plant's first line of defense against pathogens.
- Non-expressor of Pathogenesis Related 1 (NPR1) acts as the salicylic acid (SA) receptor and is vital for stomatal defense.
- The precise function of NPR1 in guard cells and its role in systemic acquired resistance (SAR) are not fully understood.
Purpose of the Study:
- To investigate the role of NPR1 in guard cell function during pathogen attack.
- To compare stomatal movement and proteomic changes in wild-type Arabidopsis and the npr1-1 mutant.
- To elucidate NPR1's contribution to systemic acquired resistance (SAR).
Main Methods:
- Comparative analysis of wild-type Arabidopsis and the npr1-1 knockout mutant.
- Assessment of stomatal movement in response to pathogen attack.
- Proteomic analysis to identify differentially abundant proteins.
Main Results:
- NPR1 does not influence stomatal density.
- The npr1-1 mutant exhibits defective stomatal closure upon pathogen attack, leading to increased pathogen ingress.
- Elevated ROS levels and altered protein abundance in carbon fixation, oxidative phosphorylation, glycolysis, and glutathione metabolism were observed in the npr1-1 mutant.
Conclusions:
- Mobile SAR signals may modulate stomatal immune responses, potentially via ROS burst initiation.
- The npr1-1 mutant displays an alternative priming effect mediated by translational regulation.
Related Concept Videos
Defenses Against Pathogens and Herbivores
23.9K
Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
23.9K
Regulation of Transpiration by Stomata
28.5K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
28.5K
Cell Signaling in Plants
5.7K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.7K
Introduction to Plant Diversity
45.2K
From Water to Land
45.2K
Adaptations that Reduce Water Loss
25.8K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
25.8K
Immune Surveillance by NK Cells and Phagocytes
1.5K
Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
1.5K


