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Determination of the molecular size of the hepatic H1-receptor by target size analysis

N P Wang, H Fukui, H Matsuoka

    Biochemical and Biophysical Research Communications
    |June 13, 1986
    PubMed

    Insights

    This study determined the molecular size of histamine H1-receptors across multiple animal species. Results indicate a consistent receptor size, with radiation affecting binding capacity but not the binding constant.

    Area of Science:

    • Pharmacology
    • Biochemistry
    • Molecular Biology

    Background:

    • Histamine H1-receptors are crucial in mediating allergic and inflammatory responses.
    • Understanding the molecular properties of these receptors is key to developing targeted therapeutics.

    Purpose of the Study:

    • To determine the molecular size of histamine H1-receptors in various animal livers.
    • To investigate the impact of radiation inactivation on receptor binding characteristics.

    Main Methods:

    • Radiation inactivation assay was employed to determine the molecular size of H1-receptors.
    • Saturation analysis was used to assess the effects of irradiation on [3H]mepyramine binding.

    Main Results:

    • Histamine H1-receptor molecular sizes ranged from 100,000 to 160,000 daltons across species.
    • Statistical analysis revealed a common hepatic H1-receptor size of 128,000 +/- 63,000 daltons.
    • Irradiation did not alter the [3H]mepyramine binding constant but decreased binding capacity.

    Conclusions:

    • Hepatic histamine H1-receptors exhibit a conserved molecular size across diverse mammalian and avian species.
    • Radiation inactivation is a valid method for assessing receptor size, and its effects on binding parameters provide insights into receptor structure-function relationships.

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