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Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

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Published on: January 7, 2017

Improving aptamer performance: key factors and strategies.

Hong Yu^1,2,3,4, Jiangxiong Zhu^1,2,3,4, Guoqing Shen^1,2,3,4

¹School of Agriculture and Biology, Key Laboratory of Urban Agriculture, Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.

Mikrochimica Acta

|June 10, 2023

Summary

Aptamers, selected via SELEX, show promise but need optimization. Post-SELEX strategies like truncation and modification enhance aptamer performance for broader applications in biosensing and therapeutics.

Keywords:

Aptamer Extension Multivalent integration Mutagenesis and modification Post-SELEX optimization Splitting Truncation

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Area of Science:

Biotechnology
Molecular Biology
Biochemistry

Background:

Aptamers are single-stranded oligonucleotides selected through Systematic Evolution of Ligands by Exponential Enrichment (SELEX).
They offer advantages over antibodies, including high specificity, low variation, and ease of synthesis.
Current aptamer performance often requires improvement for practical applications.

Purpose of the Study:

To review key factors influencing aptamer performance.
To summarize and discuss post-SELEX optimization strategies.
To highlight the importance of selecting appropriate optimization methods.

Main Methods:

Discussion of factors affecting aptamer properties.
Summary of post-SELEX optimization techniques: truncation, extension, mutagenesis, modification, splitting, and multivalent integration.
Analysis of the mechanisms behind each optimization approach.

Main Results:

Identified key factors influencing aptamer performance.
Detailed various post-SELEX optimization strategies.
Demonstrated successful improvements in aptamer performance through these methods.
Provided insights into the mechanisms of optimization.

Conclusions:

Post-SELEX optimization is crucial for enhancing aptamer utility.
A variety of methods exist to improve aptamer affinity, specificity, and stability.
Choosing the correct optimization strategy is vital for maximizing aptamer potential in diverse applications.