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Updated: Jul 27, 2025
03:28
Sperm Collection of Differential Quality Using Density Gradient Centrifugation
Published on: November 29, 2018
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Live motile sperm sorting device for enhanced sperm-fertilization competency: comparative analysis with
Cheng-Teng Hsu1, Chun-I Lee2,3,4,5, Fong-Sian Lin1
1Center for Research and Development, Bonraybio Co., Ltd, Taichung, Taiwan.
Journal of Assisted Reproduction and Genetics
|June 10, 2023
Summary
The LensHooke CA0 device offers superior sperm selection, enhancing motility and reducing DNA fragmentation compared to traditional methods. This live motile sperm sorting device demonstrates consistent efficiency for all semen sample types.
Area of Science:
- Reproductive biology
- Andrology
- Biotechnology
Background:
- Conventional sperm processing methods like density-gradient centrifugation (DGC) can negatively impact sperm quality.
- There is a need for advanced sperm selection techniques that preserve sperm viability and function.
Purpose of the Study:
- To evaluate the efficacy of the LensHooke CA0, a novel live motile sperm sorting device, against DGC and the Zymot device.
- To assess the impact of incubation time and temperature on CA0 performance.
Main Methods:
- Semen samples from 239 men were processed using CA0 under various conditions, DGC, and Zymot.
- Sperm parameters including motility, concentration, morphology, kinematics, DNA fragmentation index (DFI), and acrosome reaction rate (AR) were analyzed.
Main Results:
- CA0 processing, optimized at 30 minutes and 37°C, significantly improved total motility and motile sperm concentration.
- CA0 demonstrated superior outcomes in both normozoospermic and non-normozoospermic samples, yielding higher motility and normal morphology, and lower DFI and AR compared to DGC and Zymot.
Conclusions:
- The LensHooke CA0 device effectively selects spermatozoa with enhanced fertilization potential.
- CA0 minimizes DNA fragmentation and shows consistent, high-efficiency sperm selection for diverse semen sample qualities.
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