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Updated: Jul 27, 2025

Derivation of Mouse Trophoblast Stem Cells from Blastocysts
Published on: June 8, 2010
Derivation of trophoblast stem cells from human expanded potential stem cells
Shao Xu1, Sidong Wang2, Timothy Theodore Ka Ki Tam3
1Centre for Translational Stem Cell Biology, The University of Hong Kong, Hong Kong Special Administrative Region, China.
None:
Human trophoblast development study has long been limited by the lack of suitable materials. Here we present a detailed protocol for the differentiation of human expanded potential stem cells (hEPSCs) into human trophoblast stem cells (TSCs) and for the subsequent establishment of TSC lines. The hEPSC-derived TSC lines can be continuously passaged and are functional in further differentiation into syncytiotrophoblasts and extravillous trophoblasts. The hEPSC-TSC system offers a valuable cell source for studying human trophoblast development in pregnancy. For complete details on the use and execution of this protocol, please refer to Gao et al. (2019)1 and Ruan et al. (2022).2.
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