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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Updated: Jul 27, 2025

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
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UniProt and Mass Spectrometry-Based Proteomics-A 2-Way Working Relationship.

E H Bowler-Barnett1, J Fan1, J Luo1

  • 1European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Hinxton, United Kingdom.

Molecular & Cellular Proteomics : MCP
|June 10, 2023
PubMed
Summary
This summary is machine-generated.

The human proteome includes all proteins and their modifications. UniProtKB is a key resource for protein data, benefiting mass spectrometry researchers.

Keywords:
UniProtcomplete proteomesprotein functionprotein sequence databasesequence identifiers

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Area of Science:

  • Proteomics
  • Bioinformatics
  • Genomics

Background:

  • The human proteome encompasses all proteins, including those modified by variants and post-translational modifications.
  • UniProtKB serves as a premier resource for protein sequence and functional data.

Approach:

  • This review details the information UniProtKB provides to the mass spectrometry-based proteomics community.
  • It also highlights knowledge gained from large-scale datasets deposited by researchers.

Key Points:

  • UniProtKB offers experimentally verified and computationally predicted protein information.
  • The database integrates data from expert biocuration and public domain depositions.
  • It supports researchers in mass spectrometry-based proteomics.

Conclusions:

  • UniProtKB is a vital, freely accessible hub for human proteome data.
  • Collaboration between UniProtKB and the proteomics community enhances data quality and accessibility.