Effect of forest soil viruses on bacterial community succession and the implication for soil carbon sequestration

Although the ecological importance of soil viruses is increasingly recognized, how soil viruses regulate the diversity, structure and succession of microbial communities has not been well understood. Here, we conducted an incubation experiment by mixing soil viruses and bacteria in different ratios, and tracked the changes in viral and bacterial cell abundances as well as bacterial community composition. Our results revealed that viral predation predominantly targeted host lineages that are r-strategists and was a key regulator of the succession of bacterial communities. Viral lysis markedly enhanced production of insoluble particulate organic matter, hence potentially contributing to carbon sequestration. In addition, treatment with mitomycin C led to significant shift in virus to bacteria ratio, and revealed bacterial lineages sensitive to lysogenic-lytic conversion, e.g., Burkholderiaceae in particular, indicating effect of prophage induction on bacterial community succession. Soil viruses also promoted homogeneous selection of the bacterial communities, suggesting the role of viruses in influencing bacterial community assembly mechanisms. Overall, this study provides empirical evidence of the top-down control exerted by viruses on soil bacterial communities and expanded knowledge about the associated regulatory mechanisms.