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Genomic Sequencing to Diagnose Prosthetic Joint Infection in the Knee: A Case Report

Hollie Wilkinson1, Helen S McCarthy2,3, Jade Perry2,3

  • 1Bioengineering, Keele University, Keele, GBR.

Cureus
|June 12, 2023
PubMed

Insights

Diagnosing prosthetic joint infections (PJI) is challenging. Genomic sequencing using the MinION device offers a potentially faster and more sensitive method for bacterial identification in PJI cases compared to traditional cultures.

Area of Science:

  • Microbiology
  • Genomics
  • Medical Diagnostics

Background:

  • Prosthetic joint infections (PJI) lack a definitive diagnostic standard.
  • Current microbiological cultures for PJI diagnosis have significant limitations.
  • Accurate bacterial identification is critical for effective PJI treatment.

Observation:

  • Genomic sequencing was explored as a diagnostic tool for PJI.
  • The MinION device by Oxford Nanopore Technologies was utilized for real-time sequencing.
  • A case study involved a 61-year-old male patient with a suspected PJI.

Findings:

  • Nanopore sequencing demonstrated potential for rapid bacterial species identification in PJI.
  • Comparison with standard microbiological cultures suggested improved speed and sensitivity.
  • Genomic sequencing offers a promising alternative for diagnosing prosthetic joint infections.

Implications:

  • This approach could lead to faster PJI diagnosis and treatment initiation.
  • Real-time genomic sequencing may reduce healthcare costs associated with PJI diagnosis.
  • Further validation of nanopore sequencing for PJI diagnostics is warranted.
Keywords:
diagnosisgenomicsinfectionnanoporeorthopaedics

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