Related Experiment Video
Updated: Jul 27, 2025
12:23
In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
Published on: September 7, 2022
1.7K
Size-selective downstream processing of virus particles and non-enveloped virus-like particles
Nils Hillebrandt1, Jürgen Hubbuch1
1Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
Frontiers in Bioengineering and Biotechnology
|June 12, 2023
Summary
Size-selective separation techniques offer a promising solution for purifying non-enveloped virus-like particles (VLPs). These methods leverage size differences to efficiently remove impurities, enhancing biopharmaceutical applications.
Area of Science:
- Biotechnology
- Protein Nanoparticle Engineering
- Bioprocessing
Background:
- Non-enveloped virus-like particles (VLPs) are protein nanoparticles with significant biopharmaceutical potential.
- Conventional downstream processing (DSP) methods face challenges with VLPs due to their large size.
- Host-cell impurities often complicate VLP purification.
Purpose of the Study:
- To review the principles and applications of size-selective separation techniques for VLP purification.
- To highlight the potential of these techniques in downstream processing of virus particles (VPs).
- To discuss specific DSP steps for non-enveloped VLPs and their subunits.
Main Methods:
- Review of size-selective separation principles.
- Analysis of existing DSP techniques for VLPs.
- Exploration of size-based purification strategies.
Main Results:
- Size-selective separation effectively exploits size differences between VPs and impurities.
- These techniques demonstrate broad applicability across various VPs.
- Potential for enhanced purity and yield in VLP production.
Conclusions:
- Size-selective separation is a valuable approach for VLP downstream processing.
- These methods offer a scalable and versatile solution for biopharmaceutical applications.
- Further development can optimize VLP purification efficiency.
More Related Videos
Related Concept Videos
01:29Viruses with RNA Genomes
59
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
59
01:28Introduction to Virus
95
Viruses are unique biological entities that blur the boundary between living and non-living systems. Although they lack cellular structure and metabolic processes, they can exhibit characteristics of life when infecting a host. Their defining feature is a nucleic acid core, composed of either DNA or RNA, encapsulated within a protein coat called a capsid. This simple structure allows them to invade host cells and use their machinery for replication efficiently.Viral Structure and...
95