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Long noncoding RNA CCDC183-AS1 depletion represses breast cancer cell proliferation, colony formation, and motility

Tao Liu1, Limin Zhou1, Lianbo Zhang2

  • 1Department of Second Breast Surgery, Jilin Cancer Hospital, Changchun, 130012, China.

Oncology Research
|June 12, 2023
PubMed

Insights

Long noncoding RNA CCDC183-AS1 promotes breast cancer (BC) malignancy by regulating the miR-3918/FGFR1 axis. Targeting CCDC183-AS1 may offer new therapeutic strategies for BC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) play crucial roles in cancer development.
  • The specific role of CCDC183 antisense RNA 1 (CCDC183-AS1) in breast cancer (BC) remains largely uncharacterized.

Purpose of the Study:

  • To investigate the involvement of CCDC183-AS1 in BC malignancy.
  • To elucidate the underlying molecular mechanisms of CCDC183-AS1 in BC.

Main Methods:

  • Quantitative real-time PCR to measure CCDC183-AS1 expression.
  • Cell proliferation, colony formation, migration, and invasion assays.
  • In vivo tumor growth experiments.
  • MicroRNA (miRNA) and Western blot analyses to assess the regulatory axis.

Main Results:

  • CCDC183-AS1 expression was significantly elevated in BC tissues and correlated with poor clinical outcomes.
  • Knockdown of CCDC183-AS1 inhibited BC cell proliferation, migration, invasion, and tumor growth in vivo.
  • CCDC183-AS1 acted as a competing endogenous RNA by sponging miR-3918, leading to fibroblast growth factor receptor 1 (FGFR1) overexpression.
  • Inhibition of miR-3918 or overexpression of FGFR1 rescued the suppressive effects of CCDC183-AS1 knockdown.

Conclusions:

  • CCDC183-AS1 promotes BC cell malignancy through the CCDC183-AS1/miR-3918/FGFR1 axis.
  • CCDC183-AS1 represents a potential therapeutic target for breast cancer treatment.
Keywords:
CCDC183-AS1Long noncoding RNAceRNAmicroRNA

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