[Fluorometric method of determining the activity of ornithine decarboxylase from animal tissues]

Fluorometric determination of ornithine decarboxylase activity is described. Dansyl putrescine fluorescence on TLC-plates was used to evaluate putrescine content in the samples. The dependence of dansyl putrescine fluorescence intensity on sample putrescine content was linear in the range of 0-120 nmol. Instrumental sensitivity coefficient (SF/C = 3), relative measurement sensitivity (Cmin = 0.3 nmol per sample), and basic metrological characteristics showing high reliability, accuracy and precision of enzyme activity determination were calculated. Standard error of the mean and relative standard deviation did not exceed 2.5 and 7.5%, respectively. Increased ODC activity was found in malignant and regenerating rat liver tissue, as well as in hepatomas H-27 and 48.