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microRNA-1-3p and T-synthase mRNA have high diagnostic efficacy on intestinal mucosal barrier dysfunction in patients

Wen-Bo Wu1, Xiao-Fei Jiang1, Ming-Quan Chen1

  • 1Department of Emergency Medicine, Huashan Hospital, Fudan University, Shanghai, People's Republic of China.

The Kaohsiung Journal of Medical Sciences
|June 12, 2023
PubMed

Insights

This study found that elevated miR-1-3p and decreased T-synthase mRNA can help diagnose intestinal mucosal barrier dysfunction in severe acute pancreatitis (SAP&IBD) patients. These biomarkers are independent risk factors for SAP&IBD.

Area of Science:

  • Gastroenterology and Molecular Biology
  • Biomarker Discovery
  • Pancreatic Diseases

Background:

  • Severe acute pancreatitis (SAP) can lead to intestinal mucosal barrier dysfunction (SAP&IBD), a serious complication.
  • Effective diagnostic markers for SAP&IBD are crucial for timely intervention and improved patient outcomes.

Purpose of the Study:

  • To evaluate the diagnostic efficacy of serum miR-1-3p and T-synthase mRNA in peripheral blood B lymphocytes for identifying SAP&IBD.
  • To investigate the correlation between these biomarkers and clinical indicators of inflammation and intestinal damage.

Main Methods:

  • Serum miR-1-3p and T-synthase mRNA expression were measured in SAP patients with and without intestinal barrier dysfunction using RT-qPCR.
  • Statistical analyses included Pearson correlation, ROC curve analysis, and multivariate logistic regression.

Main Results:

  • SAP&IBD patients exhibited significantly higher serum miR-1-3p levels and lower T-synthase mRNA expression compared to SAP patients.
  • Serum miR-1-3p showed a negative correlation with T-synthase mRNA and positive correlations with Ranson score, CRP, IL-6, DAO, and D-Lactate.
  • T-synthase mRNA levels were negatively correlated with IL-6, DAO, and D-Lactate.
  • Both miR-1-3p and T-synthase mRNA, individually and in combination, demonstrated diagnostic efficiency for SAP&IBD.

Conclusions:

  • Serum miR-1-3p and T-synthase mRNA are valuable diagnostic biomarkers for intestinal mucosal barrier dysfunction in severe acute pancreatitis.
  • These biomarkers serve as independent risk factors and can aid in the diagnosis of IBD in SAP patients.
Keywords:
T-synthasediagnosisintestinal mucosal barrier dysfunctionmicroRNA-1-3psevere acute pancreatitis

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