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Related Experiment Video

Updated: Jul 26, 2025

Pancreatic Tissue Dissection to Isolate Viable Single Cells

Pancreatic Tissue Dissection to Isolate Viable Single Cells

Published on: May 26, 2023

Pancreatic Tissue Dissection to Isolate Viable Single Cells.

Oshri Yosefov-Levi¹, Sharona Tornovsky¹, Oren Parnas²

¹The Lautenberg Center for Immunology and Cancer Research, The Institute for Medical Research Israel-Canada, Faculty of Medicine, The Hebrew University of Jerusalem.

Journal of Visualized Experiments : Jove

|June 12, 2023

Summary

This summary is machine-generated.

This study presents a novel two-step protocol for pancreatic tissue digestion, crucial for isolating intact pancreatic acinar cells. The method effectively preserves cell viability, addressing a key challenge in pancreatic research.

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Last Updated: Jul 26, 2025

Pancreatic Tissue Dissection to Isolate Viable Single Cells

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Published on: May 26, 2023

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Area of Science:

Gastroenterology
Cell Biology
Biochemistry

Background:

The pancreas has endocrine and exocrine functions, with the exocrine system comprising 90% of the organ.
Pancreatic acinar cells produce digestive enzymes that can degrade cellular components like RNA.
Standard tissue dissociation methods often yield fragile cells and release damaging enzymes, hindering research.

Purpose of the Study:

To develop an improved protocol for pancreatic tissue digestion.
To enhance the recovery of intact and viable pancreatic acinar cells.
To provide a method applicable to normal, pre-malignant, and tumor pancreatic tissues.

Main Methods:

A novel two-step protocol for pancreatic tissue digestion was developed.
The protocol aims to minimize cell damage and enzyme degradation during dissociation.
The method was tested on normal, pre-malignant, and tumor pancreatic samples.

Main Results:

The developed protocol successfully recovers intact and viable pancreatic acinar cells.
This method overcomes the challenge of cell fragility and enzyme activity in pancreatic tissue.
The protocol is effective across diverse pancreatic tissue types, including those with stromal and immune cells.

Conclusions:

The two-step protocol offers a significant improvement for pancreatic tissue processing.
It enables better isolation of viable pancreatic acinar cells for downstream research.
This method is valuable for studying various pancreatic conditions, including tumors.