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Updated: Jul 26, 2025

Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors
Published on: December 11, 2021
iPSCs-Derived Neurons and Brain Organoids from Patients
Wanying Zhu1, Lei Xu1, Xinrui Li1
1School of Pharmacy, Collaborative Innovation Center for Cardiovascular Disease Translational Medicine, State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
Abstract:
Induced pluripotent stem cells (iPSCs) can be differentiated into specific neurons and brain organoids by adding induction factors and small molecules in vitro, which carry human genetic information and recapitulate the development process of human brain as well as physiological, pathological, and pharmacological characteristics. Hence, iPSC-derived neurons and organoids hold great promise for studying human brain development and related nervous system diseases in vitro, and provide a platform for drug screening. In this chapter, we summarize the development of the differentiation techniques for neurons and brain organoids from iPSCs, and their applications in studying brain disease, drug screening, and transplantation.
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