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Updated: Jul 26, 2025

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
Published on: July 26, 2022
Glycosylation in extracellular vesicles: Isolation, characterization, composition, analysis and clinical applications
Veronika Vrablova1, Natalia Kosutova1, Anna Blsakova1
1Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, Bratislava 845 38, Slovak Republic.
Abstract:
This review provides a comprehensive overview of our understanding of the role that glycans play in the formation, loading and release of extracellular vesicles (EVs). The capture of EVs (typically with a size of 100-200 nm) is described, including approaches based on glycan recognition with glycan-based analysis offering highly sensitive detection of EVs. Furthermore, detailed information is provided about the use of EV glycans and glycan processing enzymes as potential biomarkers, therapeutic targets or tools applied for regenerative medicine. The review also provides a short introduction into advanced methods for the characterization of EVs, new insights into the biomolecular corona covering EVs and bioanalytical tools available for glycan analysis.
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