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Association of IFIH1 and DDX58 genes polymorphism with susceptibility to COVID-19

Parisa Feizollahi1,2, Mohammad Hossein Zamanian3, Sara Falahi1

  • 1Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Daneshgah Street, Shahid Shiroudi Boulevard, PO-Box: 6714869914, Kermanshah, Iran.

Medical Microbiology and Immunology
|June 12, 2023
PubMed

Insights

Genetic variations in the DDX58 gene, specifically the rs10813831 polymorphism, are linked to increased COVID-19 susceptibility. This innate immune system study highlights a potential genetic factor influencing disease severity in Iran.

Area of Science:

  • Immunology
  • Genetics
  • Virology

Background:

  • Pattern recognition receptors (RLRs) like RIG-I and MDA5 are crucial for innate immunity and viral detection.
  • Genetic variations in RLRs may influence COVID-19 severity.
  • RLR signaling plays a role in immune responses.

Purpose of the Study:

  • To investigate the association between specific single nucleotide polymorphisms (SNPs) in the IFIH1 and DDX58 genes and COVID-19 susceptibility.
  • To analyze the impact of these genetic variations on disease severity in the Kermanshah population, Iran.

Main Methods:

  • Genotyping of three SNPs (rs1990760 and rs3747517 in IFIH1; rs10813831 in DDX58) using PCR-RFLP.
  • Analysis of genomic DNA from 177 severe and 182 mild COVID-19 patients.
  • Statistical analysis of genotype frequencies and their association with COVID-19 susceptibility.

Main Results:

  • The AA genotype of DDX58 rs10813831 (G>A) was significantly associated with increased COVID-19 susceptibility (p=0.017, OR=2.593).
  • A significant association was also observed in the recessive model for rs10813831 (AA vs. GG+GA, p=0.003, OR=2.901).
  • No significant association was found between IFIH1 gene polymorphisms (rs1990760 and rs3747517) and COVID-19.

Conclusions:

  • The DDX58 rs10813831 polymorphism may be associated with COVID-19 severity in the studied Iranian population.
  • IFIH1 gene polymorphisms do not appear to be associated with COVID-19 susceptibility in this cohort.
  • Further research is warranted to elucidate the role of DDX58 in COVID-19 pathogenesis.
Keywords:
MDA-5RIG-ISARS-CoV-2Single-nucleotide polymorphisms

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