[Effects of Biochar Application on Soil Bacterial Community Diversity and Winter Wheat Growth in Wheat Fields]

A long-term field experiment was conducted to study the diversity of soil bacterial communities and the response of crop growth to biochar application, in order to provide a scientific basis for the rational application of biochar in agricultural fields. Four treatments were applied at 0 (B0 blank), 5 (B1), 10 (B2), and 20 t·hm^-2(B3) to investigate the effects of biochar on soil physical and chemical properties, soil bacterial community diversity, and growth of winter wheat using Illumina MiSeq high-throughput sequencing technology. The results showed that soil water content, pH value, soil organic carbon, total nitrogen, nitrate nitrogen content, winter wheat biomass, nitrogen uptake, and yield showed an increasing trend with the increase in biochar amount. The high-throughput sequencing results showed that the B2 treatment significantly reduced the alpha diversity of the bacterial community at the flowering stage. The overall response of soil bacterial community composition to different application rates of biochar and phenological phases was taxonomically consistent. In this study, Proteobacteria, Acidobacteria, Planctomycetes, Gemmatimonadetes, and Actinobacteria were the dominant bacterial phyla. The relative abundance of Acidobacteria decreased, but the relative abundance of Proteobacteria and Planctomycetes increased with biochar application. The results of redundancy analysis, co-occurrence network analysis, and PLS-PM analysis indicated that bacterial community compositions were closely associated with soil parameters such as soil nitrate and total nitrogen. The average connectivity between 16S OTUs was higher under the B2 and B3 treatments (16.966 and 14.600) than under the B0 treatment. The variation in soil bacterial community (89.1%) was regulated by biochar and sampling period and partly explained the changes in the growth dynamics of winter wheat (0.077). In conclusion, biochar application could regulate the changes in the soil bacterial community and promote crop growth after seven years of application. It is suggested that 10-20 t·hm^-2 biochar should be applied in semi-arid agricultural areas to achieve sustainable agricultural development.