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Production of Humanized Mice through Stem Cell Transfer
Cameron Phelps1, Devra D Huey1, Stefan Niewiesk1
1Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio.
Current Protocols
|June 13, 2023
Summary
Humanized mice, created using human umbilical cord stem cells in immunodeficient mice, are valuable for studying human diseases. Protocols for generating and analyzing these humanized mice in the NSG background are detailed.
Area of Science:
- Immunology
- Translational Medicine
- Animal Models
Background:
- Humanized mice are critical for studying human diseases in vivo.
- Severely immunodeficient mouse strains are essential for successful human cell engraftment.
- Human umbilical cord stem cells are a key component for creating humanized models.
Purpose of the Study:
- To present established protocols for generating humanized mice.
- To detail methods for analyzing humanized mouse models.
- To provide a guide for researchers utilizing the NSG mouse background.
Main Methods:
- Engraftment of human umbilical cord stem cells into neonatal and 4-week-old immunodeficient mice.
- Preparation of human umbilical cord stem cells for transplantation.
- Analysis of peripheral blood in humanized mice via flow cytometry following submandibular blood collection.
Main Results:
- Successful engraftment and development of human lymphocytes in immunodeficient mice.
- Demonstrated feasibility of humanized mouse generation using NSG background.
- Established methods for sample collection and analysis.
Conclusions:
- The presented protocols enable reliable generation and analysis of humanized mice.
- Humanized mice serve as a powerful platform for translational research in human diseases.
- These methods facilitate the study of human immune system development and function in vivo.
