Covalent Organic Framework Based Crosslinked Porous Microcapsules for Enzymatic Catalysis
Mengchu Feng1, Ziru Niu1, Chunyan Xing1
1Frontiers Science Center for High Energy Material, Advanced Technology Research Institute (Jinan), Key Laboratory of Cluster Science (Ministry of Education), Beijing Key Laboratory of Photoelectronic/Electrophotonic Conversion Materials, Advanced Research Institute of Multidisciplinary Science, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, 100081, P. R. China.
We developed novel porous microcapsules using covalent organic framework (COF) spheres for enzyme encapsulation. These robust COF microcapsules enhance enzyme activity and stability in organic solvents, advancing biocatalysis applications.
Area of Science:
- Materials Science
- Chemical Engineering
- Biotechnology
Background:
- Enzyme encapsulation in robust, selectively permeable microcapsules is crucial for biocatalysis.
- Current methods face challenges in achieving both mechanical stability and controlled mass transfer.
Purpose of the Study:
- To develop a facile method for fabricating porous microcapsules for enzyme encapsulation.
- To investigate the properties and performance of these microcapsules in biocatalytic applications.
Main Methods:
- Fabrication of porous microcapsules via self-assembly of covalent organic framework (COF) spheres at emulsion interfaces.
- Interparticle crosslinking of COF spheres to enhance structural integrity.
- Enzyme encapsulation within the COF microcapsules.
Main Results:
- Successfully synthesized porous COF microcapsules with size-selective shells.
- Demonstrated enhanced enzyme activity and durability in organic media.
- Verified performance in both batch and continuous-flow biocatalytic reactions.
Conclusions:
- The developed COF microcapsules provide a stable, selective platform for enzyme encapsulation.
- This technology shows significant potential for improving biocatalysis efficiency and enzyme longevity.
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