Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fibroblasts: a diverse population of cells balancing homeostasis, wound healing, regeneration, inflammation, fibrosis, and cancer across organs.

JCI insight·2026
Same author

Efficacy and Safety of Oteseconazole in Severe Vulvovaginal Candidiasis Categorized by <i>Candida</i> Species and Previous Genital Tract Infection: A Post Hoc Analysis of a Phase 3 Trial.

Drug design, development and therapy·2026
Same author

Circulating CTHRC1 levels are associated with IPF disease severity and survival.

Thorax·2026
Same author

Lung adventitial fibroblasts support type 2 Tregs to shape the response to influenza infection.

bioRxiv : the preprint server for biology·2026
Same author

Ultra-large targeted DNA integrations in primary human cells.

bioRxiv : the preprint server for biology·2026
Same author

DROP-CARs: Engineering Reversible, Drug-Controlled CAR T-cell Activity with a Clinically Approved Small Molecule.

Cancer research·2026

Related Experiment Video

Updated: Jul 26, 2025

A Method of Targeted Cell Isolation via Glass Surface Functionalization
10:40

A Method of Targeted Cell Isolation via Glass Surface Functionalization

Published on: September 20, 2016

9.5K

An immune-based tool platform for in vivo cell clearance.

Jieqiong Zhang1, Tatsuya Tsukui2, Xiumin Wu3

  • 1Calico Life Sciences LLC, South San Francisco, CA, USA zjq.thu@gmail.com.

Life Science Alliance
|June 13, 2023
PubMed
Summary

Researchers developed a flexible platform to label cells with ovalbumin (OVA) for targeted elimination. This approach successfully targeted hepatocytes and, using T cells, reduced fibrosis by eliminating pro-fibrotic fibroblasts.

More Related Videos

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
10:13

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

9.0K
Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments
07:46

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

Published on: April 30, 2021

4.8K

Related Experiment Videos

Last Updated: Jul 26, 2025

A Method of Targeted Cell Isolation via Glass Surface Functionalization
10:40

A Method of Targeted Cell Isolation via Glass Surface Functionalization

Published on: September 20, 2016

9.5K
Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
10:13

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

9.0K
Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments
07:46

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

Published on: April 30, 2021

4.8K

Area of Science:

  • Immunology
  • Cellular Biology
  • Pathobiology

Background:

  • Immunological targeting is a proven strategy in oncology and is being explored for other diseases.
  • Developing methods to eliminate specific pathological cell types is crucial for treating various conditions.

Purpose of the Study:

  • To present a versatile platform for labeling and eliminating specific cell populations using immunological approaches.
  • To evaluate the efficacy of this platform in targeting hepatocytes and pro-fibrotic fibroblasts.

Main Methods:

  • Cells of interest were labeled with the model antigen ovalbumin (OVA) on their surface.
  • Targeted elimination was achieved using either antigen-specific T cells or novel OVA antibodies.
  • The platform's effectiveness was tested in targeting hepatocytes and fibroblasts in a pulmonary fibrosis model.

Main Results:

  • Hepatocytes were effectively eliminated by both T cells and OVA antibodies.
  • Pro-fibrotic fibroblasts were eliminated by T cells, leading to reduced collagen deposition in a fibrosis model.
  • Initial experiments showed differential targeting capabilities based on cell type and elimination modality.

Conclusions:

  • The developed platform offers a flexible method for immunological cell targeting.
  • This system can be utilized to eliminate pathological cell types, including pro-fibrotic fibroblasts, in vivo.
  • The platform holds promise for advancing immune-based therapies for various diseases.