Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

<i>Populus</i>-Inspired Aerogel Evaporator Based on Vertically Oriented Porous Structure for Efficient Solar Steam Generation.

Langmuir : the ACS journal of surfaces and colloids·2026

Same author

Straw ash-derived black carbon particles: Insights into potassium release and transport mechanisms.

Waste management (New York, N.Y.)·2026

Same author

An investigation into internet gaming disorder and psychological correlates among medical undergraduates in vocational colleges in China: a cross-sectional appraisal.

BMC psychiatry·2026

Same author

Differences in gut microbiota composition are an important reason for lower serum p-cresol sulfate levels in anuric peritoneal dialysis patients compared to hemodialysis patients.

Current research in microbial sciences·2026

Same author

Identification of thermotolerant non-canonical PAMs for robust one-pot CRISPR-Cas12a detection.

Nature communications·2026

Same author

Variation in microbiome and metabolites is associated with advantageous effects of cholestyramine on primary biliary cholangitis with pruritus.

Microbiology spectrum·2026

Search research articles

Related Experiment Video

Updated: Jul 26, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

Chain hybridization-based CRISPR-lateral flow assay enables accurate gene visual detection.

Meng Cheng¹, Caiwei Tan¹, Bo Xiang¹

¹Department of Laboratory Medicine, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Analytica Chimica Acta

|June 13, 2023

Summary

A new CRISPR-CHLFA platform overcomes false positives in gene detection assays. This nucleic acid hybridization method offers accurate, visualized point-of-care testing for infectious diseases.

Keywords:

Accuracy CRISPR Chain hybridization Lateral flow assay Visual detection

More Related Videos

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays

Published on: January 7, 2019

Related Experiment Videos

Last Updated: Jul 26, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays

Published on: January 7, 2019

Area of Science:

Biotechnology
Molecular Biology
Diagnostics

Background:

CRISPR-Cas gene detection using lateral flow assays (CRISPR-LFA) shows promise for point-of-care testing.
Conventional CRISPR-LFA methods often yield false-positive results due to reliance on immuno-based test strips.

Purpose of the Study:

To develop a novel CRISPR-based lateral flow assay (CHLFA) that eliminates false positives.
To establish a highly accurate and visualized gene detection platform for point-of-care applications.

Main Methods:

Developed a Chain Hybridization-based Lateral Flow Assay (CHLFA) platform utilizing nucleic acid hybridization.
Integrated CRISPR-Cas12a or CRISPR-Cas13a with GNP-probe hybridization, bypassing the need for immunoreactions.
Assayed for target gene detection within 50 minutes, with sensitivity down to 1-10 gene copies.

Main Results:

The CRISPR-CHLFA system successfully eliminated false-positive results common in conventional CRISPR-LFA.
Achieved highly accurate visual detection of target-negative samples.
Demonstrated 100% accuracy in detecting SARS-CoV-2 Omicron variant and Mycobacterium tuberculosis in clinical specimens.

Conclusions:

The CRISPR-CHLFA platform provides a robust alternative for accurate, visualized gene detection.
This technology is suitable for developing advanced point-of-care testing (POCT) biosensors.
CHLFA offers a significant improvement over existing CRISPR-LFA methods for disease diagnostics.