L-Theanine Improves Locomotor Function in a Model of Multiple Sclerosis Mice

Area of Science:

• Neuroscience
• Pharmacology

Background:

• Multiple Sclerosis (MS) is a debilitating neurological disease.
• Cuprizone (CPZ) is widely used to induce experimental MS models in rodents.
• Oxidative stress plays a significant role in MS pathogenesis.

Purpose of the Study:

• To investigate the neuroprotective potential of L-theanine in a mouse model of MS.
• To evaluate the effects of L-theanine on motor function and oxidative stress markers in CPZ-induced MS.

Main Methods:

• Male C57BL/6 mice were divided into four groups: control, CPZ-induced MS, and CPZ-induced MS treated with L-theanine.
• Mice received either a control diet, a CPZ-containing diet, or a CPZ-containing diet supplemented with L-theanine (50mg/kg).
• Motor behavior (ambulation, grip strength, etc.) and serum antioxidant levels (MDA, SOD, GPx, TAS) were assessed.

Main Results:

• CPZ administration significantly impaired motor behavior and increased oxidative stress (elevated MDA, decreased SOD, GPx, TAS).
• L-theanine treatment in CPZ-fed mice ameliorated motor deficits and normalized antioxidant status.
• L-theanine significantly improved ambulation, grip strength, and other motor functions while reducing MDA and boosting antioxidant enzymes.

Conclusions:

• L-theanine exhibits significant neuroprotective effects against CPZ-induced experimental MS in mice.
• L-theanine mitigates motor dysfunction and oxidative stress, suggesting its therapeutic potential for MS.