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Updated: Aug 1, 2026
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
Identification and Characterization of a Vancomycin Intermediate-Resistant Staphylococcus haemolyticus Isolated from
Wanyang Dong1, Qi Peng1, Xiaohua Tang1,2
1Guangzhou Key Laboratory for Clinical Rapid Diagnosis and Early Warning of Infectious Diseases, KingMed School of Laboratory Medicine, Guangzhou Medical University, Guangzhou, 510180, People's Republic of China.
Background:
Staphylococcus haemolyticus is an opportunistic pathogen that belongs to coagulase-negative Staphylococci (CoNS). Increasing infection and multi-drug resistance cases caused by this strain have been reported and thus it poses a great health threat.
Methods:
The third-generation sequencing technology was performed on a S. haemolyticus SH-1 isolated from a clinical sample to analyze the drug resistance genes, which included vancomycin resistance related genes. In addition, antimicrobial susceptibility tests, transmission electron microscopy and Triton X-100 stimulated autolysis were conducted to understand its biological characteristics.
Results:
The study shows that this clinical isolate is a vancomycin intermediate-resistant strain. Genome comparison also revealed that WalK(N70K) and WalK(R280Q) mutations may contribute to the vancomycin resistant phenotype. Besides, S. haemolyticus SH-1 exhibit common features of thicker cell wall and decreased autolytic activity.
Conclusion:
S. haemolyticus SH-1 with WalKR mutations shows typical characteristics of vancomycin resistant strains. Combining the genome features and biological properties, our findings may provide important information for the understanding of the molecular mechanism of S. haemolyticus to vancomycin intermediate-resistance.
Insights
This study identifies vancomycin intermediate-resistance in Staphylococcus haemolyticus SH-1, linked to specific WalKR mutations. These findings offer insights into the molecular mechanisms driving resistance in this opportunistic pathogen.
Area of Science:
- Microbiology
- Genomics
- Molecular Biology
Background:
- Staphylococcus haemolyticus is an opportunistic pathogen within the coagulase-negative Staphylococci group.
- Increasing infections and multi-drug resistance cases highlight its significant public health threat.
Purpose of the Study:
- To analyze drug resistance genes, including vancomycin resistance, in a clinical S. haemolyticus isolate.
- To investigate the biological characteristics associated with vancomycin intermediate-resistance.
Main Methods:
- Third-generation sequencing for genome analysis.
- Antimicrobial susceptibility testing.
- Transmission electron microscopy and autolysis assays.
Main Results:
- The S. haemolyticus SH-1 isolate demonstrated vancomycin intermediate-resistance.
- WalK(N70K) and WalK(R280Q) mutations were identified and may contribute to vancomycin resistance.
- The strain exhibited a thicker cell wall and reduced autolytic activity.
Conclusions:
- WalKR mutations in S. haemolyticus SH-1 correlate with vancomycin resistance characteristics.
- The study provides valuable information on the molecular mechanisms of vancomycin intermediate-resistance in S. haemolyticus.
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