LncRNA THRIL Functions as a Marker for Carotid Artery Stenosis and Affects the Biological Function of Human Aortic
Fei Wang1, Ying Wang2, Xichun Guo3
1Department of Neurosurgery, Affiliated Hospital of Weifang Medical University, Weifang, People's Republic of China.
Insights
Long non-coding RNA THRIL is elevated in carotid artery restenosis (CAS) and predicts poor prognosis. Down-regulating THRIL impacts endothelial cell proliferation, apoptosis, and inflammation, suggesting its role in CAS pathogenesis.
Area of Science:
- Vascular Biology
- Molecular Medicine
- Biomarker Discovery
Background:
- Carotid artery restenosis (CAS) is a significant cause of cerebrovascular disease and mortality.
- Understanding the molecular mechanisms underlying CAS is crucial for developing effective diagnostic and therapeutic strategies.
Purpose of the Study:
- To evaluate the predictive value of long non-coding RNA (lncRNA) TNFalpha-and hnRNP L-related immunoregulatory lncRNA (THRIL) in CAS.
- To investigate the association of THRIL with the pathogenesis of CAS.
Main Methods:
- THRIL expression was measured in patients with asymptomatic CAS and in human aortic endothelial cells (HAECs) exposed to oxidized low-density lipoprotein (ox-LDL).
- Receiver operating characteristic (ROC) curves and Kaplan-Meier (K-M) analysis were used for prognosis prediction.
- Cell proliferation, apoptosis, and inflammation were assessed using MTT, flow cytometry, and ELISA.
Main Results:
- THRIL expression was significantly higher in patients with CAS.
- ROC analysis confirmed THRIL's predictive potential for CAS.
- K-M and Cox regression revealed THRIL expression and CAS severity as independent risk factors for poor prognosis.
- THRIL was upregulated in ox-LDL-induced HAECs, and its downregulation promoted proliferation, inhibited apoptosis, and reduced inflammation.
Conclusions:
- THRIL serves as a diagnostic and prognostic biomarker for CAS.
- THRIL plays a critical role in regulating endothelial cell behavior, including proliferation, apoptosis, and inflammation, in the context of ox-LDL exposure.
Purpose:
Carotid artery restenosis (CAS) is a leading contributor to cerebrovascular diseases and one of the leading causes of death in the world. The purpose of this study was to assess the predictive efficiency of long non-coding RNA (lncRNA) TNFalpha-and hnRNP L-related immunoregulatory lncRNA (THRIL) and its association with the pathogenesis of CAS.
Patients And Methods:
The expression of THRIL was determined in patients with asymptomatic CAS and human aortic endothelial cell (HAEC) models induced by oxidized low-density lipoprotein (ox-LDL). The receiver operating characteristic (ROC) curve and Kaplan-Meier (K-M) drawings were constructed to predict the risk of poor prognosis in patients with CAS. The cell proliferation, death rate, and inflammation were detected by 3-(4,5-dimethyl-2-thiazyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), flow cytometry, and enzyme-linked immunosorbent assay (ELISA) assays.
Results:
The relative expression of THRIL was elevated in patients with asymptomatic CAS. The findings of ROC curve indicated that THRIL had a predictive possibility on CAS. K-M finding and Cox regression analysis showed that the expression of THRIL and the degree of CAS were independent risk factors for poor prognosis in patients with CAS. THRIL was up-expressed in HAECs induced by ox-LDL. Down-regulation of THRIL could promote the proliferation of HAECs, inhibit cell apoptosis, and restrict cell inflammation.
Conclusion:
THRIL was a diagnostic and prognostic biomarker in CAS and played an important role in regulating the proliferation, apoptosis, and inflammation of HAECs induced by ox-LDL.
Related Concept Videos
02:39lncRNA - Long Non-coding RNAs
01:30Atherosclerosis I: Introduction
01:19Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...