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Updated: Jul 26, 2025
Modeling Neonatal Intraventricular Hemorrhage Through Intraventricular Injection of Hemoglobin
Published on: August 25, 2022
[Circular RNA hsa_circ_0087893 participates in intraventricular hemorrhage occurrence and progression possibly as a
1Department of Neonatology, General Hospital of Ningxia Medical University, Yinchuan 750000, China.
Insights
Circular RNAs (circRNAs) were screened in preterm infants with intraventricular hemorrhage (IVH). The circRNA hsa_circ_0087893 may act as a ceRNA, impacting IVH development in premature infants.
Area of Science:
- Biochemistry
- Genetics
- Neonatal Medicine
Context:
- Intraventricular hemorrhage (IVH) is a significant complication in preterm infants.
- Circular RNAs (circRNAs) are emerging as key regulators in various biological processes.
- Understanding the molecular mechanisms underlying IVH is crucial for improving neonatal outcomes.
Purpose:
- To identify differentially expressed circRNAs in the serum of preterm infants with IVH.
- To investigate the competitive endogenous RNA (ceRNA) mechanism involving circRNAs in IVH.
- To explore the potential role of hsa_circ_0087893 in the pathogenesis of IVH.
Summary:
- A circRNA array identified 121 differentially expressed circRNAs in preterm infants with IVH.
- Gene Ontology and pathway analyses revealed involvement in cell proliferation, DNA repair, and metabolism.
- hsa_circ_0087893 was significantly downregulated and formed a network with miRNAs and mRNAs, suggesting a ceRNA role.
Impact:
- This study highlights the potential of circRNAs as biomarkers for IVH in preterm neonates.
- The findings provide insights into the ceRNA network's involvement in IVH pathogenesis.
- hsa_circ_0087893 emerges as a potential therapeutic target for managing IVH in premature infants.
Objective:
To screen for differentially expressed circular RNAs (circRNAs) in the serum of preterm infants with intraventricular hemorrhage (IVH) and explore the competitive endogenous RNA (ceRNA) mechanism of circRNAs in IVH in these infants.
Methods:
Fifty preterm infants (gestational age of 28 to 34 weeks) admitted in our department between January, 2019 and January, 2020 were enrolled in this study, including 25 with a MRI diagnosis of IVH and 25 without IVH. Serum samples were collected from 3 randomly selected infants from each group for profiling differentially expressed circRNAs using circRNA array technique. Gene ontology (GO) and pathway analyses were performed to reveal the function of the identified circRNAs. The circRNA-miRNA-mRNA network was constructed to identify the co-expression network of hsa_circ_ 0087893.
Results:
A total of 121 differentially expressed circRNAs were identified in the infants with IVH, including 62 up-regulated and 59 down-regulated circRNAs. GO and pathway analyses showed that these circRNAs were involved in multiple biological processes and pathways, including cell proliferation, activation and death, DNA damage and repair, retinol metabolism, sphingolipid metabolism, cell adhesion molecules. Among these circRNAs, hsa_circ_0087893 was found to have significant down-regulation in IVH group and co-express with 41 miRNAs and 15 mRNAs (such as miR-214-3p, miR-761, miR-183-5p, AKR1B1, KRT34, PPP2CB, and HPRT1).
Conclusion:
The circRNA hsa_circ_0087893 may function as a ceRNA and play an important role in the occurrence and progression of IVH in preterm infants.
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