Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

5.8K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
5.8K
Flow Cytometry01:23

Flow Cytometry

13.2K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
13.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic blood compatibility assessment of coronary stents using an innovative pneumatic blood pump to generate physiological flow rates in small sample chambers.

Journal of materials science. Materials in medicine·2025
Same author

Continuous microfluidic flow-through protocol for selective and image-activated electroporation of single cells.

RSC advances·2023
Same author

Micropatterned Thermoresponsive Cell Culture Substrates for Dynamically Controlling Neurite Outgrowth and Neuronal Connectivity in Vitro.

ACS applied bio materials·2022
Same author

Combining dielectrophoresis and computer vision for precise and fully automated single-cell handling and analysis.

Lab on a chip·2019

Related Experiment Video

Updated: Jul 26, 2025

Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells
13:44

Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells

Published on: November 10, 2023

2.0K

High-precision, low-complexity, high-resolution microscopy-based cell sorting.

Tobias Gerling1,2, Neus Godino1, Felix Pfisterer1

  • 1Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses IZI-BB, Am Muehlenberg 13, 14476 Potsdam, Germany. michael.kirschbaum@izi-bb.fraunhofer.de.

Lab on a Chip
|June 14, 2023
PubMed
Summary

This study introduces a low-complexity microfluidic system for high-resolution image-activated cell sorting, enabling efficient isolation of specific cell types with high purity and vitality.

More Related Videos

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
14:30

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Published on: August 7, 2021

6.7K
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.7K

Related Experiment Videos

Last Updated: Jul 26, 2025

Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells
13:44

Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells

Published on: November 10, 2023

2.0K
Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
14:30

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Published on: August 7, 2021

6.7K
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.7K

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Microfluidics

Background:

  • Image-activated cell sorting (IACS) leverages cellular features for isolating specialized cells.
  • Current IACS methods face limitations in image quality and experimental complexity, hindering widespread adoption.

Purpose of the Study:

  • To develop a simplified, high-performance IACS system.
  • To improve image resolution and processing time for enhanced cell sorting accuracy.
  • To demonstrate the system's capability in isolating viable T cells based on subcellular localization.

Main Methods:

  • A novel microfluidic device integrating high numerical aperture wide-field microscopy.
  • Precise dielectrophoretic cell manipulation for controlled handling.
  • Advanced image analysis allowing extended processing times (hundreds of milliseconds).

Main Results:

  • Achieved unprecedented image resolution of 216 nm in IACS.
  • Successfully sorted live T cells based on subcellular fluorescence signals with >80% purity.
  • Demonstrated high cell recovery rate (85%) and confirmed cell vitality post-sorting.

Conclusions:

  • The developed microfluidic approach offers a low-complexity, high-resolution solution for IACS.
  • This system overcomes previous limitations, making IACS a more accessible and powerful tool.
  • The method ensures high purity, yield, and vitality of sorted cells, advancing cell isolation technologies.