Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

5.5K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
5.5K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

830
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
830
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

1.5K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.5K
Mass Spectrometry: Molecular Fragmentation Overview01:20

Mass Spectrometry: Molecular Fragmentation Overview

3.3K
The ionization of a molecule into a molecular ion inside the mass spectrometer causes instability in the molecule's structure due to the loss of an electron. This eventually leads to the fragmentation or breaking of some bonds in the molecule. The fragmentation occurs predominantly at specific bonds to yield relatively stable fragments.
One type of fragmentation pattern is the cleavage of a single bond in the molecular ion. The cleavage leads to a radical and a cation. The cleavage can...
3.3K
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

1.3K
An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a low-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.
To...
1.3K
Mass Spectrometers01:16

Mass Spectrometers

5.7K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hetairos is a histology-based artificial intelligence model for predicting central nervous system tumor methylation subtypes.

Nature cancer·2026
Same author

Humics-Functionalized Iron(III) Oxyhydroxides as Promising Nanoferrotherapeutics: Synthesis, Characterization, and Efficacy in Iron Delivery.

Nanomaterials (Basel, Switzerland)·2025
Same author

The fluorescence asymmetry ratio as an optical index of antioxidant activity of RP-HPLC fractions of fulvic acids.

Analytical methods : advancing methods and applications·2025
Same author

Integrated in vivo combinatorial functional genomics and spatial transcriptomics of tumours to decode genotype-to-phenotype relationships.

Nature biomedical engineering·2025
Same author

Segger: Fast and accurate cell segmentation of imaging-based spatial transcriptomics data.

bioRxiv : the preprint server for biology·2025
Same author

Onboard Large-Scale Isolation and Characterization of Three Reference DOM Materials from Siberian Arctic Shelf Marine Water.

ACS omega·2025

Related Experiment Video

Updated: Jul 26, 2025

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

8.7K

NOMspectra: An Open-Source Python Package for Processing High Resolution Mass Spectrometry Data on Natural Organic

Alexander Volikov1, Gleb Rukhovich1, Irina V Perminova1

  • 1Department of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia.

Journal of the American Society for Mass Spectrometry
|June 14, 2023
PubMed
Summary

NOMspectra is a new Python package that simplifies processing complex high-resolution mass spectrometry data for natural organic matter (NOM) and humic substances (HS). It offers tools for analysis, visualization, and elemental composition assignment, making complex data more accessible.

More Related Videos

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules
09:59

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

Published on: March 5, 2014

19.0K
Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy
11:47

Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy

Published on: April 7, 2012

12.9K

Related Experiment Videos

Last Updated: Jul 26, 2025

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

8.7K
MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules
09:59

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

Published on: March 5, 2014

19.0K
Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy
11:47

Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy

Published on: April 7, 2012

12.9K

Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry
  • Computational Chemistry

Background:

  • Natural organic matter (NOM) and humic substances (HS) are complex mixtures.
  • High-resolution mass spectrometry (HRMS) generates intricate data patterns for NOM/HS.
  • Processing these complex HRMS datasets requires specialized analytical methods.

Purpose of the Study:

  • Introduce NOMspectra, a Python package for HRMS data analysis of NOM.
  • Provide a comprehensive workflow for processing, analyzing, and visualizing NOM/HS mass spectra.
  • Enhance accessibility and user-friendliness for researchers in the field.

Main Methods:

  • Development of a Python package, NOMspectra.
  • Implementation of algorithms for spectral filtering and recalibration.
  • Inclusion of functions for elemental composition assignment and molecular descriptor calculation.
  • Integration of data visualization tools and a graphical user interface (GUI).

Main Results:

  • NOMspectra offers a robust workflow for handling complex NOM/HS HRMS data.
  • The package facilitates spectral processing, recalibration, and elemental composition assignment.
  • Includes tools for calculating molecular descriptors and advanced data visualization.
  • A user-friendly GUI enhances the package's accessibility.

Conclusions:

  • NOMspectra provides an integrated solution for analyzing complex natural organic matter mass spectrometry data.
  • The package democratizes advanced HRMS data analysis for NOM and HS research.
  • Facilitates deeper understanding of NOM composition and properties through accessible computational tools.