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Updated: Jul 26, 2025
3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
Published on: September 13, 2018
SCARA5 as a downstream factor of PCAT29, inhibits proliferation, migration, and invasion of bladder cancer
Xin-Sheng Lu1, Meng-Long Huang1, Li-Bo Chen1
1The First Affiliated Hospital, Department of Urology, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China.
Abstract:
Scavenger receptor class A, member 5 (SCARA5) has been identified a novel tumor suppressor in several cancers. However, the functional and underlying mechanism of SCARA5 in bladder cancer (BC) need investigation. Here, we found SCARA5 expression was downregulated in both BC tissues and cell lines. Low SCARA5 in BC tissues was associated with a shorter overall survival. Moreover, SCARA5 overexpression reduced BC cell viability, colony formation, invasion, and migration. Further investigation demonstrated that the expression of SCARA5 was negatively regulated by miR-141. Furthermore, the long non-coding RNA prostate cancer associated transcript 29 (PCAT29) inhibited the proliferation, invasion, and migration of BC cells by sponging miR-141. Luciferase activity assays revealed that PCAT29 targeted miR-141 and miR-141 targeted SCARA5. In conclusion, SCARA5, as a downstream factor of the PCAT29/miR-141 axis, inhibited the proliferation, migration, and invasion of BC cells. These findings provide novel insights into the detailed molecular mechanisms of BC development.
Insights
Scavenger receptor class A, member 5 (SCARA5) acts as a tumor suppressor in bladder cancer (BC). The PCAT29/miR-141 axis regulates SCARA5, impacting BC cell proliferation and invasion.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Scavenger receptor class A, member 5 (SCARA5) is a recognized tumor suppressor in various cancers.
- The specific role and molecular mechanisms of SCARA5 in bladder cancer (BC) remain largely unexplored.
Purpose of the Study:
- To investigate the functional role and regulatory mechanisms of SCARA5 in bladder cancer.
- To elucidate the molecular pathway involving SCARA5, miR-141, and PCAT29 in BC development.
Main Methods:
- Analysis of SCARA5 expression in BC tissues and cell lines.
- Assessment of SCARA5 overexpression effects on BC cell behavior (viability, colony formation, invasion, migration).
- Luciferase activity assays to confirm targeting interactions between PCAT29, miR-141, and SCARA5.
Main Results:
- SCARA5 expression was significantly downregulated in BC tissues and cell lines.
- Lower SCARA5 levels correlated with reduced overall survival in BC patients.
- SCARA5 overexpression suppressed BC cell proliferation, colony formation, invasion, and migration.
- PCAT29 acted as a competing endogenous RNA (ceRNA) for miR-141, which in turn targets SCARA5, establishing the PCAT29/miR-141/SCARA5 regulatory axis.
Conclusions:
- SCARA5 functions as a tumor suppressor in bladder cancer, inhibiting proliferation, migration, and invasion.
- The long non-coding RNA PCAT29 influences BC progression by sponging miR-141, thereby regulating SCARA5 expression.
- This study reveals a novel molecular mechanism underlying bladder cancer development, highlighting the PCAT29/miR-141/SCARA5 pathway.
