Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Three-dimensional reconstruction from serial sections. IV. The reassembly problem.

J S Prothero, J W Prothero

    Computers and Biomedical Research, an International Journal
    |August 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A model of cell cleavage.

    Biophysical journal·2009
    Same author

    Three-dimensional reconstruction from serial sections. V. Calibration of dimensional changes incurred during tissue preparation and data processing.

    Analytical and quantitative cytology and histology·1991
    Same author

    Department size and quality.

    Science (New York, N.Y.)·1990
    Same author

    Three-dimensional contour surfacing of the skull, face, and brain from CT and MR images and from anatomic sections.

    AJR. American journal of roentgenology·1988
    Same author

    Evidence for a universal process underlying clonal attenuation.

    Mechanisms of ageing and development·1987
    Same author

    TOPPER, a software package in FORTRAN for scaling studies.

    International journal of bio-medical computing·1985

    This study addresses the challenge of creating accurate 3D biological models from serial sections. It presents a technical solution to the complex reassembly problem, enabling precise digital reconstruction of microscopic structures.

    Area of Science:

    • Biology
    • Medicine
    • Biomedical Engineering

    Background:

    • Quantitative descriptions of biological structures at micrometer resolution are crucial in biology and medicine.
    • Three-dimensional (3D) reconstruction from serial sections is the primary method for achieving this resolution.
    • Current methods involve complex preliminary steps and data processing challenges.

    Purpose of the Study:

    • To address the non-trivial reassembly problem in 3D reconstruction from serial sections.
    • To provide a technical description and mathematical solution for accurately combining digitized coordinates.
    • To facilitate the generation of faithful digital representations of biological objects for morphometric analysis.

    Main Methods:

    • Fixation, embedding, fiducial introduction, serial sectioning, and staining of biological tissues.

    Related Experiment Videos

  • Photomicrography or videomicrography of adjacent fields within serial sections.
  • Manual digitization of contours from projected images and subsequent computational reassembly.

    • Main Results:

    • A technical description of the 3D reconstruction reassembly problem is presented.
    • Key factors influencing the solution of the reassembly problem are discussed.
    • A mathematical statement outlining a solution to the reassembly problem is provided.

    Conclusions:

    • Accurate 3D reconstruction of biological structures from serial sections is feasible with a robust solution to the reassembly problem.
    • The presented mathematical framework offers a pathway to numerically faithful digital representations.
    • This work supports advanced quantitative morphometrics in biological and medical research.