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Updated: Jul 26, 2025

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

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Published on: April 7, 2014

Thermo-optical measurements using quantitative phase microscopy.

Nicholaus Kevin Tanjaya, Keiichiro Toda, Takuro Ideguchi

|June 15, 2023

Summary

This summary is machine-generated.

This study introduces a new method using quantitative phase microscopy (QPM) to measure thermal conductivity and the thermo-optic coefficient (TOC) of transparent materials. The technique offers a simpler approach for material characterization.

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Area of Science:

Optics and Photonics
Materials Science
Thermal Engineering

Background:

Quantitative phase microscopy (QPM) visualizes phase shifts in transparent materials.
Laser heating can alter these phase shifts, offering a potential measurement avenue.
Accurate characterization of thermal properties is crucial for material applications.

Purpose of the Study:

To simultaneously determine the thermal conductivity and thermo-optic coefficient (TOC) of transparent substrates.
To develop a QPM-based method for efficient material property measurement.
To validate the method's accuracy and potential for broader applications.

Main Methods:

Utilizing a quantitative phase microscopy (QPM) setup with an external heating laser.
Coating transparent substrates with a thin titanium nitride film for photothermal heat generation.
Employing semi-analytical modeling based on heat transfer and thermo-optic effects to extract material properties.

Main Results:

Simultaneous measurement of thermal conductivity and TOC was achieved.
The extracted thermal conductivity and TOC values showed reasonable agreement.
The method demonstrated potential for characterizing various transparent substrates.

Conclusions:

The developed QPM-based technique provides a concise and effective method for simultaneous thermal property determination.
The simple modeling and setup offer advantages over existing techniques.
This approach holds promise for characterizing the thermal conductivity and TOC of diverse transparent materials.