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Effects of erythromycin, josamycin and spiramycin on rat polymorphonuclear leukocyte chemotaxis
Abstract:
The effects of three macrolide antibiotics were studied on rat polymorphonuclear leukocyte chemotaxis. Rats were given 25 mg/kg twice a day of either erythromycin, josamycin or spiramycin by gastric intubation for 5 days. In all cases, chemotaxis was found to be impaired by 10-20% only. As macrolides are known to reach high intracellular concentrations within polymorphonuclear leukocytes, our results suggest that these antibiotics are unlikely to exert a deleterious influence on the chemotactic response of treated patients.
Insights
Three macrolide antibiotics showed minimal impact on rat polymorphonuclear leukocyte chemotaxis. This suggests erythromycin, josamycin, and spiramycin are unlikely to harm patient chemotactic responses.
Area of Science:
- Immunology
- Pharmacology
- Microbiology
Background:
- Polymorphonuclear leukocytes (PMNs) are crucial for immune response.
- Macrolide antibiotics achieve high intracellular concentrations in PMNs.
- Potential impact of macrolides on PMN function requires investigation.
Purpose of the Study:
- To evaluate the effects of erythromycin, josamycin, and spiramycin on rat PMN chemotaxis.
- To determine if macrolide treatment impairs the immune cell migration.
Main Methods:
- Rats received 25 mg/kg of erythromycin, josamycin, or spiramycin twice daily for 5 days via gastric intubation.
- Chemotaxis of polymorphonuclear leukocytes was measured.
- Antibiotic effects on cell migration were quantified.
Main Results:
- A modest impairment of 10-20% in PMN chemotaxis was observed across all three macrolides.
- No significant deleterious effect on chemotactic response was noted.
- Results indicate a minimal functional impact on PMN migration.
Conclusions:
- Macrolide antibiotics like erythromycin, josamycin, and spiramycin have a limited effect on PMN chemotaxis.
- The findings suggest that clinical use of these macrolides is unlikely to compromise patient immune cell function.
- Further research could explore long-term effects or different immune parameters.