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Bismuth release from endodontic materials: in vivo analysis using Wistar rats
M A Marciano1, L E Pelepenko1, T M Francati1
1Piracicaba Dental School of Dentistry, University of Campinas, Piracicaba, Brazil.
Scientific Reports
|June 15, 2023
Summary
Bismuth was released from calcium silicate-based materials, accumulating locally and systemically, primarily in kidneys, regardless of silicon presence. This indicates potential bismuth migration and accumulation in peripheral organs.
Area of Science:
- Biomaterials science
- Dental materials
- Toxicology
Background:
- Calcium silicate-based materials are crucial for root canal therapy, interfacing with periodontal tissues.
- Potential for elemental release and systemic distribution necessitates investigation.
Purpose of the Study:
- To evaluate bismuth release from ProRoot MTA and its accumulation in peripheral organs.
- To assess systemic and local elemental migration over 30 and 180 days in an animal model.
Main Methods:
- Scanning electron microscopy/energy dispersive spectroscopy (SEM/EDS), X-ray diffraction, micro X-ray fluorescence, and Raman spectroscopy for material and elemental analysis.
- Histological analysis for tissue response.
- Inductively coupled plasma mass spectrometry (ICP-MS) for elemental quantification in tissues and blood.
Main Results:
- Bismuth was detected locally at implantation sites and systemically in kidneys, blood, liver, and brain.
- Kidneys showed the highest bismuth accumulation compared to other organs.
- No significant changes in routine blood analysis or biochemical tests were observed.
Conclusions:
- Bismuth is released from ProRoot MTA and migrates both locally and systemically.
- The kidneys are a primary site for bismuth accumulation.
- The null hypothesis regarding bismuth migration from silicon-associated materials was rejected.
