Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Oscillating dissipative structures in mitochondrial suspensions.

E L Holmuhamedov

    European Journal of Biochemistry
    |August 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Adaphostin and other anticancer drugs quench the fluorescence of mitochondrial potential probes.

    Cell death and differentiation·2005
    Same author

    Mitochondria: gateway for cytoprotection.

    Circulation research·2001
    Same author

    Restoration of Ca2+-inhibited oxidative phosphorylation in cardiac mitochondria by mitochondrial Ca2+ unloading.

    Molecular and cellular biochemistry·2001
    Same author

    Increased calcium vulnerability of senescent cardiac mitochondria: protective role for a mitochondrial potassium channel opener.

    Mechanisms of ageing and development·2001
    Same author

    Diazoxide protects mitochondria from anoxic injury: implications for myopreservation.

    The Journal of thoracic and cardiovascular surgery·2001
    Same author

    Mitochondrial K(ATP) channels: probing molecular identity and pharmacology.

    Journal of molecular and cellular cardiology·2000
    Same journal

    Comparison of expression patterns and cell adhesion properties of the mouse biliary glycoproteins Bgp1 and Bgp2.

    European journal of biochemistry·2020
    Same journal

    AB 3.1.1.1 (or EC 3.1.1.?).

    European journal of biochemistry·2020
    Same journal

    Cdk5.

    European journal of biochemistry·2018
    Same journal

    Structure of the core oligosaccharide of a rough-type lipopolysaccharide of Pseudomonas syringae pv. phaseolicola.

    European journal of biochemistry·2004
    Same journal

    Monitoring ligand-mediated nuclear receptor-coregulator interactions by noncovalent mass spectrometry.

    European journal of biochemistry·2004
    Same journal

    Solution structure of long neurotoxin NTX-1 from the venom of Naja naja oxiana by 2D-NMR spectroscopy.

    European journal of biochemistry·2004
    See all related articles

    Spatial structures form in unstirred layers of oscillating mitochondria. These patterns synchronize with mitochondrial oscillations and are suppressed by respiratory chain inhibitors, revealing new insights into mitochondrial dynamics.

    Area of Science:

    • Biophysics
    • Cell Biology
    • Biochemistry

    Background:

    • Mitochondria exhibit oscillatory behavior crucial for cellular energy production.
    • The unstirred layer surrounding biological structures can influence observed phenomena.
    • Understanding mitochondrial dynamics is key to cellular health and disease.

    Purpose of the Study:

    • To investigate the occurrence and characteristics of spatial structures in the unstirred layer of oscillating mitochondria.
    • To determine the relationship between these spatial structures and mitochondrial oscillations.
    • To explore the factors affecting these spatial patterns, including layer depth and respiratory chain activity.

    Main Methods:

    • Observation of spatial structures using a photo camera and light scattering.

    Related Experiment Videos

  • Analysis of light scattering patterns in the unstirred layer of mitochondrial suspension.
  • Correlation of observed patterns with mitochondrial oscillation periods.
  • Assessment of the effect of varying layer depth (0.3-3.0 mm) on patterning.
  • Evaluation of the impact of respiratory chain inhibitors (antimycin A, CN-) on spatial patterning.

    • Main Results:

    • Spatial structures were detected in the unstirred layer of oscillating mitochondrial suspensions.
    • These structures oscillated with the same period as the bulk mitochondrial oscillations.
    • Patterning was independent of layer depth within the tested range.
    • Different oscillatory states of mitochondria corresponded to distinct spatial patterns.
    • Inhibitors of the respiratory chain, antimycin A and CN-, effectively suppressed patterning.

    Conclusions:

    • Oscillating mitochondria generate synchronized spatial structures in their unstirred layer.
    • These patterns serve as indicators of mitochondrial oscillatory states.
    • Respiratory chain activity is essential for the formation of these spatial structures.
    • The findings offer a novel method for studying mitochondrial dynamics and function.